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Leptospiral immunoglobulin-like proteins elicit protective immunity.
- Source :
-
Vaccine [Vaccine] 2004 Mar 29; Vol. 22 (11-12), pp. 1545-52. - Publication Year :
- 2004
-
Abstract
- The identification of new vaccine candidates for leptospirosis is of great interest. This paper describes the identification of two homologous immunogenic proteins, LigA-m and LigB-m, of Leptospira interrogans serovar manilae strain UP-MMC-NIID, which show strong similarity with LigA found recently in L. interrogans serovar pomona. Southern blot analysis indicated the conservation of the lig genes among pathogenic leptospires. LigA-m protein was shown to be a surface-exposed lipoprotein expressed during in vitro culture. Both LigA-m and LigB-m induced protective immunity against a challenge with a homologous serovar of L. interrogans in mice. Furthermore, patients' sera infected with heterologous serovars' strains contained antibodies against LigA-m and LigB-m. Our results suggest that LigA-m and LigB-m proteins are new vaccine candidates for the prevention of leptospirosis.
- Subjects :
- Animals
Antigens, Bacterial immunology
Blotting, Southern
Electrophoresis, Polyacrylamide Gel
Endopeptidase K
Enzyme-Linked Immunosorbent Assay
Escherichia coli genetics
Humans
Hydrolysis
Mice
Mice, Inbred C3H
Plasmids genetics
Plasmids immunology
Recombinant Proteins immunology
Reverse Transcriptase Polymerase Chain Reaction
Vaccines, Synthetic immunology
Bacterial Proteins immunology
Immunoglobulins immunology
Leptospira interrogans immunology
Leptospirosis immunology
Leptospirosis prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 0264-410X
- Volume :
- 22
- Issue :
- 11-12
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 15063580
- Full Text :
- https://doi.org/10.1016/j.vaccine.2003.10.007