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Aurora B -TACC1 protein complex in cytokinesis.
- Source :
-
Oncogene [Oncogene] 2004 Jun 03; Vol. 23 (26), pp. 4516-22. - Publication Year :
- 2004
-
Abstract
- Taxins are a family of centrosomal proteins important for the regulation of mitosis and microtubule dynamics. Cytokinesis, the last step of M phase, is essential for chromosomal integrity and cell division. It is highly regulated and involves a reorganization of microtubules and actin filaments. We show here that TACC1 localizes diffusely to the midzone spindle in anaphase and strongly to the midbody during cytokinesis, indicating a possible involvement of this protein in the exit of M phase. TACC1 also relocalizes to the nucleolus in interphase. We demonstrate that TACC1 and the mitotic kinase Aurora B belong to the same complex during cytokinesis. We further show that Aurora B knocked down by RNA-mediated interference prevents the formation of the midbody - and consequently affects TACC1 localization at this site - and leads to abnormal cell division and multinucleated cells.
- Subjects :
- Anaphase
Aurora Kinase B
Aurora Kinases
Cell Division physiology
Cell Nucleus genetics
Fetal Proteins genetics
HeLa Cells
Humans
Macromolecular Substances
Microtubule-Associated Proteins genetics
Mitosis physiology
Nuclear Proteins genetics
Protein Serine-Threonine Kinases genetics
Protein Transport
RNA Interference
Spindle Apparatus metabolism
Fetal Proteins metabolism
Microtubule-Associated Proteins metabolism
Nuclear Proteins metabolism
Protein Serine-Threonine Kinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0950-9232
- Volume :
- 23
- Issue :
- 26
- Database :
- MEDLINE
- Journal :
- Oncogene
- Publication Type :
- Academic Journal
- Accession number :
- 15064709
- Full Text :
- https://doi.org/10.1038/sj.onc.1207593