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alpha-Melanocyte-stimulating hormone, MSH 11-13 KPV and adrenocorticotropic hormone signalling in human keratinocyte cells.
- Source :
-
The Journal of investigative dermatology [J Invest Dermatol] 2004 Apr; Vol. 122 (4), pp. 1010-9. - Publication Year :
- 2004
-
Abstract
- alpha-MSH signals by binding to the melanocortin-1 receptor (MC-1R) and elevating cyclic AMP in several different cells. The anti-inflammatory properties of this peptide are also believed to be cyclic AMP dependent. The carboxyl terminal tripeptides of alpha-MSH (KPV / KP-D-V) are the smallest minimal sequences reported to prevent inflammation but it is not known if they operate via MC-1R or cyclic AMP. The aim of this study was to examine the intracellular signalling of key MSH and ACTH peptides in human keratinotocytes. No elevation in cyclic AMP was detected in either HaCaT or normal human keratinocytes in response to alpha-MSH, KPV or ACTH peptides. Rapid and acute intracellular calcium, however, were observed in HaCaT keratinocytes in response to alpha-MSH (10(-15)-10(-7) M), KPV (10(-15)-10(-7) M), KP-D-V (10(-15)-10(-7) M) and ACTH (10(-15)-10(-7) M), but only in the presence of PIA, an adenosine agonist that inhibits the cyclic AMP pathway. Normal keratinocytes responded to all the above peptides but in addition responded to ACTH 1-17 (10(-13)-10(-7) M) in contrast to the HaCaT keratinocytes. Stable transfection of Chinese hamster ovary cells with the MC-1 receptor showed that alpha-MSH and the KPV peptides elevated intracellular calcium.
- Subjects :
- Adrenocorticotropic Hormone pharmacology
Animals
CHO Cells
Calcium metabolism
Cells, Cultured
Cricetinae
Cricetulus
Cyclic AMP metabolism
Humans
Intracellular Membranes metabolism
Melanocyte-Stimulating Hormones pharmacology
Monophenol Monooxygenase metabolism
NF-kappa B physiology
Peptide Fragments pharmacology
Receptor, Melanocortin, Type 1 metabolism
alpha-MSH pharmacology
Adrenocorticotropic Hormone metabolism
Keratinocytes metabolism
Melanocyte-Stimulating Hormones metabolism
Peptide Fragments metabolism
Signal Transduction
alpha-MSH metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0022-202X
- Volume :
- 122
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The Journal of investigative dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 15102092
- Full Text :
- https://doi.org/10.1111/j.0022-202X.2004.22404.x