[A rapid procedure to purify serum IgG from Microtus fotis].

Objective: To evaluate the procedure to purify IgG antibodies from Microtus fotis serum.
Methods: IgG antibodies from sera of three groups of Microtus fotis were purified by protein G or protein A affinity chromatography, their purity and binding capacity were compared.
Results: The protein G affinity chromatography was more efficient than protein A affinity chromatography. The antibodies isolated from protein G affinity chromatography showed a higher purity and better activity than that from protein A affinity chromatography monitored by SDS-PAGE and ELISA. The ability of the purified IgG to bind the second antibodies were 8.5 times and 3.1 times that of non-IgG proteins and unpurified sera, respectively.
Conclusion: The protein G affinity chromatography is a rapid, convenient and reliable procedure for Microtus fotis serum IgG purification.