[Fusion expression of Neisseria gonorrhoeae outmembrane protein with a mucosal adjuvant].

Objective: To express a fusion protein of Neisseria gonorrhoeae with a mucosal adjuvant.
Methods: The gene coding Loop VI-VIII(PL678) of porin, an out-membrane protein of Neisseria gonorrhoeae, was obtained by PCR. It was inserted into a plasmid fused with subunit B of heat labile enterotoxin. The recombinant was transformed in E. coli. The expression of fusion protein was analysed by ELISA, SDS-PAGE and Western-blot.
Result: Fusion protein with LTB was successfully expressed, and displayed both the ability of binding GM1 and the reactogenicity with polyclonal antibodies against Neisseria gonorrhoeae.
Conclusion: The expression of fusion protein laid a foundation for the study of the intramolecular vaccine against Neisseria gonorrhoeae.