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Biolocalisation and photochemical properties of two novel macrocyclic photosensitisers: a spectroscopic study.
- Source :
-
Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2004 May 27; Vol. 74 (2-3), pp. 73-84. - Publication Year :
- 2004
-
Abstract
- Biolocalisation and photochemical properties of novel macrocyclic photosensitisers, guanidiniocarbonyl-substituted tetraphenylporphyrin (1) and sugar-substituted sapphyrin (2) were investigated by spectroscopic methods. Both photosensitisers absorb in far visible region and showed good tumour localisation. Photosensitiser 2 demonstrated significantly larger absolute and relative to normal tissue (T/N) amount in tumour (330 microg g(-1) wet tissue, T/N=19.0) than photosensitiser 1 did (13 microg g(-1) wet tissue, T/N=2.1). According to iodometric and uric acid assays, compound 1 produced large amount of 1O2 (phidelta=0.60-0.68), while compound 2 showed non-significant 1O2 production (phidelta=0.04). The electronic spectroscopic study confirms that only photosensitiser 1 is able to mediate photooxidation of model compounds (BSA, poly(Trp), Tyr, Trp, and GMP) after light irradiation. Pour photochemical activity of compound 2 was explained by its self-aggregation. Raman spectroscopic study indicated that monomerised photosensitiser 2 effectively damaged BSA and calf thymus DNA after light excitation at the conditions of high excess of these macromolecules.
- Subjects :
- Absorption
Animals
Carbohydrates chemistry
DNA chemistry
DNA metabolism
DNA radiation effects
Female
Mice
Molecular Structure
Neoplasms metabolism
Neoplasms pathology
Oxygen metabolism
Photochemistry
Photosensitizing Agents administration & dosage
Porphyrins administration & dosage
Serum Albumin, Bovine chemistry
Serum Albumin, Bovine metabolism
Solutions chemistry
Spectrum Analysis
Spectrum Analysis, Raman
Guanine chemistry
Photosensitizing Agents chemistry
Photosensitizing Agents pharmacokinetics
Porphyrins chemistry
Porphyrins pharmacokinetics
Subjects
Details
- Language :
- English
- ISSN :
- 1011-1344
- Volume :
- 74
- Issue :
- 2-3
- Database :
- MEDLINE
- Journal :
- Journal of photochemistry and photobiology. B, Biology
- Publication Type :
- Academic Journal
- Accession number :
- 15157902
- Full Text :
- https://doi.org/10.1016/j.jphotobiol.2004.02.003