Back to Search
Start Over
Visualisation of zebrafish infection by GFP-labelled Vibrio anguillarum.
- Source :
-
Microbial pathogenesis [Microb Pathog] 2004 Jul; Vol. 37 (1), pp. 41-6. - Publication Year :
- 2004
-
Abstract
- Vibrio anguillarum is an invasive pathogen of fish causing a septicaemia called vibriosis. In this work, transparent zebrafish were immersed in water containing green fluorescent protein labelled V. anguillarum. The infection was visualised at the whole fish and single bacterium levels using microscopy. The gastrointestinal tract was the first site where the pathogen was detected. This enteric localisation occurred independently of the flagellum or motility. On the other hand, chemotactic motility was essential for association of the pathogen with the fish surface. In conclusion, the zebrafish infection model provides evidence that the intestine and skin represent sites of infection by V. anguillarum and suggests a host site where chemotaxis may function in virulence.
- Subjects :
- Animals
Bacterial Adhesion
Chemotaxis
Flagella physiology
Gastrointestinal Tract microbiology
Gastrointestinal Tract pathology
Genes, Reporter
Green Fluorescent Proteins
Luminescent Proteins genetics
Movement
Skin microbiology
Skin pathology
Vibrio physiology
Vibrio Infections pathology
Luminescent Proteins metabolism
Staining and Labeling methods
Vibrio pathogenicity
Vibrio Infections microbiology
Zebrafish microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 0882-4010
- Volume :
- 37
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Microbial pathogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 15194159
- Full Text :
- https://doi.org/10.1016/j.micpath.2004.03.001