Back to Search
Start Over
Hepatocyte growth factor protects small airway epithelial cells from apoptosis induced by tumor necrosis factor-alpha or oxidative stress.
- Source :
-
Pediatric research [Pediatr Res] 2004 Sep; Vol. 56 (3), pp. 336-44. Date of Electronic Publication: 2004 Jun 16. - Publication Year :
- 2004
-
Abstract
- Involvement of hepatocyte growth factor (HGF) in lung morphogenesis and regeneration has been established by in vitro and in vivo experiments in animals. In the present study, the protective activity of HGF against tumor necrosis factor (TNF)-alpha or hydrogen peroxide (H2O2)-induced damage of pulmonary epithelial cells was examined using the human small airway epithelial cell line (SAEC). Western blot analysis revealed that the receptor for HGF (c-Met) was highly expressed on the surface of SAEC and its downstream signal transduction pathway was functional. The SAEC was induced into apoptosis by the treatment with TNF-alpha or H2O2 in a dose-dependant manner, but was significantly rescued from apoptosis in the presence of HGF. The HGF effect was evident when added not only at the same time but also within several hours after treatment. This protective activity of HGF against the TNF-alpha- or H2O2-induced apoptosis was mediated, at least in part, by up-regulating the nuclear factor kappaB activity and an increase in the ratio of apoptosis-suppressing to apoptosis-inducing proteins. These results suggest that administration of HGF might exhibit a potent function in vivo for protection and improvement of acute and chronic lung injuries induced by inflammation and/or oxidative stress.
- Subjects :
- Adult
Animals
Cell Line
Cell Shape
Cell Survival
Dose-Response Relationship, Drug
Epithelial Cells cytology
Humans
Hydrogen Peroxide pharmacology
Male
NF-kappa B metabolism
Oxidants pharmacology
Proto-Oncogene Proteins c-bcl-2 metabolism
Proto-Oncogene Proteins c-met metabolism
bcl-2-Associated X Protein
bcl-X Protein
Apoptosis physiology
Epithelial Cells drug effects
Epithelial Cells metabolism
Hepatocyte Growth Factor metabolism
Oxidative Stress
Respiratory Mucosa cytology
Tumor Necrosis Factor-alpha pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0031-3998
- Volume :
- 56
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Pediatric research
- Publication Type :
- Academic Journal
- Accession number :
- 15201405
- Full Text :
- https://doi.org/10.1203/01.PDR.0000134255.58638.59