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Role of TAP-1 and/or TAP-2 antigen presentation defects in tumorigenicity of mouse melanoma.
- Source :
-
Cellular immunology [Cell Immunol] 2004 Apr; Vol. 228 (2), pp. 130-7. - Publication Year :
- 2004
-
Abstract
- Mutations in transporters associated with antigen processing (TAP-1 and -2) required for the transport of cytosolic endogenous peptides to the endoplasmic reticulum correlate with increased metastatic potential and reduced host survival in several malignancies. To address the possible function of TAP as a "tumor suppressor" gene, we show that correction of TAP-1 and/or TAP-2 defects in B16 mouse melanoma enhanced the cell surface expression of MHC class I molecules and significantly reduced the rate of subcutaneous tumor growth and pulmonary metastatic burden. Cytotoxic assays confirmed increased sensitivity of TAP-1 and/or TAP-2 transfected clones of B16 melanoma to cytotoxic T lymphocytes. These results indicate that the expression of TAP limits the malignant potential of tumors with implications for CD8(+) T cell-based immunotherapy in controlling growth of certain TAP-deficient malignancies.
- Subjects :
- ATP Binding Cassette Transporter, Subfamily B, Member 2
ATP Binding Cassette Transporter, Subfamily B, Member 3
ATP-Binding Cassette Transporters genetics
Animals
Antigen Presentation genetics
Blotting, Northern
Cytotoxicity Tests, Immunologic
Flow Cytometry
Histocompatibility Antigens Class I immunology
Immunotherapy
Lung Neoplasms genetics
Lung Neoplasms pathology
Male
Melanoma, Experimental genetics
Melanoma, Experimental pathology
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
RNA, Neoplasm chemistry
RNA, Neoplasm genetics
T-Lymphocytes, Cytotoxic
Transfection
ATP-Binding Cassette Transporters immunology
Antigen Presentation immunology
Lung Neoplasms immunology
Melanoma, Experimental immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0008-8749
- Volume :
- 228
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Cellular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 15219464
- Full Text :
- https://doi.org/10.1016/j.cellimm.2004.04.006