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Identification of novel keratinocyte-secreted peptides dermokine-alpha/-beta and a new stratified epithelium-secreted protein gene complex on human chromosome 19q13.1.
- Source :
-
Genomics [Genomics] 2004 Aug; Vol. 84 (2), pp. 384-97. - Publication Year :
- 2004
-
Abstract
- We performed high-throughput in situ hybridization screening of sections of mouse epidermis using an equalized skin cDNA library as probes and identified a novel gene giving rise to two splicing variants, both of which are expressed in the spinous layer. This gene was mapped between two genes encoding keratinocyte-related peptides, suprabasin and keratinocyte differentiation-associated protein (Kdap), on human chromosome 19q13.1. These gene products appeared to carry functional signal sequences. We then designated these two splicing variants as dermokine-alpha and -beta. Northern blotting and quantitative RT-PCR revealed that dermokine-alpha/-beta, suprabasin, and Kdap were highly expressed in stratified epithelia. In mouse embryonic development, dermokine-alpha/-beta began to be expressed during the period of stratification. Also, in differentiating primary cultured human keratinocytes, transcription of dermokine-alpha/-beta, suprabasin, and Kdap was induced. These findings indicated that dermokine-alpha/-beta, suprabasin, and Kdap are secreted from the spinous layer of the stratified epithelia and that these genes form a novel gene complex on the chromosome.
- Subjects :
- Amino Acid Sequence
Animals
Antigens, Differentiation metabolism
Aspartic Acid Endopeptidases metabolism
Cloning, Molecular
Epidermis metabolism
Exons genetics
Gene Expression Profiling
Humans
In Situ Hybridization
Intercellular Signaling Peptides and Proteins
Mice
Molecular Sequence Data
Multigene Family genetics
Physical Chromosome Mapping
Proteins chemistry
Proteins metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
Sequence Alignment
Chromosomes, Human, Pair 19 genetics
Keratinocytes metabolism
Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0888-7543
- Volume :
- 84
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Genomics
- Publication Type :
- Academic Journal
- Accession number :
- 15234001
- Full Text :
- https://doi.org/10.1016/j.ygeno.2004.03.010