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Cytotoxicity of denture base resins: effect of water bath and microwave postpolymerization heat treatments.
- Source :
-
The International journal of prosthodontics [Int J Prosthodont] 2004 May-Jun; Vol. 17 (3), pp. 340-4. - Publication Year :
- 2004
-
Abstract
- Purpose: This study compared the effect of two postpolymerization heat treatments on the cytotoxicity of three denture base resins on L929 cells using 3H-thymidine incorporation and MTT assays.<br />Materials and Methods: Sample disks of Lucitone 550, QC 20, and Acron MC resins were fabricated under aseptic conditions and stored in distilled water at 37 degrees C for 48 hours. Specimens were then divided into three groups: (1) heat treated in microwave oven for 3 minutes at 500 W; (2) heat treated in water bath at 55 degrees C for 60 minutes; and (3) no heat treatment. Eluates were prepared by placing three disks into a sterile glass vial with 9 mL of Eagle's medium and incubating at 37 degrees C for 24 hours. The cytotoxic effect from the eluates was evaluated using the 3H-thymidine incorporation and MTT assays, which reflect DNA synthesis levels and cell metabolism, respectively.<br />Results: The components leached from the resins were cytotoxic to L929 cells when 3H-thymidine incorporation assay was employed. In contrast, eluates from all resins revealed noncytotoxic effects as measured by MTT assay. For both MTT assay and 3H-thymidine incorporation, the heat treatments did not decrease the cytotoxicity of the materials tested.<br />Conclusion: Resins were graded by 3H-thymidine incorporation assay as slightly cytotoxic and by MTT assay as noncytotoxic. Cytotoxicity of the denture base materials was not influenced by microwave or water bath heat treatment.
Details
- Language :
- English
- ISSN :
- 0893-2174
- Volume :
- 17
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The International journal of prosthodontics
- Publication Type :
- Academic Journal
- Accession number :
- 15237883