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Prevalence and distribution of HSV-1, VZV, and HHV-6 in human cranial nerve nuclei III, IV, VI, VII, and XII.
- Source :
-
Journal of medical virology [J Med Virol] 2004 Sep; Vol. 74 (1), pp. 102-6. - Publication Year :
- 2004
-
Abstract
- The etiology of idiopathic cranial nerve palsies often remains unresolved. It has been hypothesised that viral reactivation of herpesviruses in the corresponding nuclei in the brainstem is the cause. We investigated the distribution of herpes simplex virus type 1 (HSV-1) and varicella zoster virus (VZV) in nuclei that are associated with peripheral sensory ganglia [oculomotor (nIII), facial (nVII) nuclei] and in nuclei that are not associated with peripheral sensory ganglia [trochlear (nIV), abducens (nVI), and hypoglossal (nXII) nuclei] of five human brainstems. Samples of the cranial nerve nuclei and adjacent control tissue were taken from histological sections after precise identification of every single nucleus and control tissue. DNA and RNA amplification methods were used to determine the prevalence and distribution of HSV-1 and VZV. The distribution of human herpes virus type 6 (HHV-6) was also determined and served as a control, since HHV-6 infection has never been associated with idiopathic cranial nerve palsies. HSV-1 was distributed at random in all cranial nerve nuclei and control tissue, whereas VZV DNA was not detected in any of the samples examined. Surprisingly, HHV-6 was present in almost all samples where HSV-1 was also present, however, the latency associated transcript (LAT) of HSV-1 was not found in any of the samples positive for HSV-1 DNA. The absence of LAT in the samples positive for HSV-1 and the distribution of HSV-1 and HHV-6 do not support the hypothesis that idiopathic cranial nerve palsies result from viral reactivation in the brainstem nuclei.
- Subjects :
- Abducens Nerve virology
Adult
Aged
Cranial Nerve Diseases etiology
DNA, Viral analysis
Facial Nerve virology
Female
Ganglia virology
Humans
Hypoglossal Nerve virology
Male
MicroRNAs
Nucleic Acid Amplification Techniques
Oculomotor Nerve virology
RNA, Viral analysis
Trochlear Nerve virology
Viral Proteins genetics
Virus Activation
Brain Stem virology
Cranial Nerves virology
Herpesvirus 1, Human isolation & purification
Herpesvirus 3, Human isolation & purification
Herpesvirus 6, Human isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 0146-6615
- Volume :
- 74
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of medical virology
- Publication Type :
- Academic Journal
- Accession number :
- 15258975
- Full Text :
- https://doi.org/10.1002/jmv.20152