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Liver-expressed chemokine/CC chemokine ligand 16 attracts eosinophils by interacting with histamine H4 receptor.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2004 Aug 01; Vol. 173 (3), pp. 2078-83. - Publication Year :
- 2004
-
Abstract
- Liver-expressed chemokine (LEC)/CCL16 is a human CC chemokine that is constitutively expressed by the liver parenchymal cells and present in the normal plasma at high concentrations. Previous studies have shown that CCL16 is a low-affinity ligand for CCR1, CCR2, CCR5, and CCR8 and attracts monocytes and T cells. Recently, a novel histamine receptor termed type 4 (H4) has been identified and shown to be selectively expressed by eosinophils and mast cells. In this study, we demonstrated that CCL16 induced pertussis toxin-sensitive calcium mobilization and chemotaxis in murine L1.2 cells expressing H4 but not those expressing histamine receptor type 1 (H1) or type 2 (H2). CCL16 bound to H4 with a K(d) of 17 nM. By RT-PCR, human and mouse eosinophils express H4 but not H3. Accordingly, CCL16 induced efficient migratory responses in human and mouse eosinophils. Furthermore, the responses of human and mouse eosinophils to CCL16 were effectively suppressed by thioperamide, an antagonist for H3 and H4. Intravenous injection of CCL16 into mice induced a rapid mobilization of eosinophils from bone marrow to peripheral blood, which was also suppressed by thioperamide. Collectively, CCL16 is a novel functional ligand for H4 and may have a role in trafficking of eosinophils.
- Subjects :
- Animals
Bone Marrow drug effects
Calcium Signaling drug effects
Cell Line drug effects
Chemokines, CC physiology
Eosinophils cytology
Eosinophils metabolism
Evolution, Molecular
Humans
Leukocyte Count
Ligands
Liver metabolism
Mice
Pertussis Toxin pharmacology
Phylogeny
Piperidines pharmacology
Protein Binding
Receptors, Cell Surface classification
Receptors, G-Protein-Coupled antagonists & inhibitors
Receptors, G-Protein-Coupled physiology
Receptors, Histamine physiology
Receptors, Histamine H3 drug effects
Receptors, Histamine H4
Recombinant Proteins pharmacology
Chemokines, CC pharmacology
Chemotaxis, Leukocyte drug effects
Eosinophils drug effects
Receptors, G-Protein-Coupled drug effects
Receptors, Histamine drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 173
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 15265943
- Full Text :
- https://doi.org/10.4049/jimmunol.173.3.2078