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Growth impairment of primary chondrocyte cells by serum of rats with chronic renal failure.
- Source :
-
Experimental & molecular medicine [Exp Mol Med] 2004 Jun 30; Vol. 36 (3), pp. 243-50. - Publication Year :
- 2004
-
Abstract
- Insulin-like growth factor (IGF)/IGF binding protein (IGFBP) abnormalities may be important in the pathogenesis of growth failure in chronic renal failure (CRF). We induced experimental CRF by 5/6 nephrectomy in Sprague Dawley rats (100 g) and observed for 2 weeks comparing with sham-operated pair-fed control rats (Sham- C). CRF rats gained 30% less height than Sham- C rats (P < 0.01). Serum IGFBP profiles by Western ligand blot revealed that IGFBP4 was elevated two fold in CRF rats (P < 0.01 vs. Sham-C). However, IGFBP4 mRNA levels in liver or skeletal muscle were not different in two groups. To determine if the increase of serum IGFBP4 in CRF retarded the growth of cartilage, epiphyseal chondrocytes were isolated from CRF or control rats and cultured in the presence of control or CRF rat sera. Incubation with 10% CRF serum reduced proliferations of normal chondrocytes and L6 rat skeletal muscle cells. In contrast, 10% CRF serum did not inhibit the growth of CRF chondrocytes. Rat sera from two groups were separated into two different fractions, high (>10 kDa, containing IGFBPs) and low (<10 kDa, containing free IGF) molecular weight fractions using a gel filtration column. Both fractions obtained from CRF sera decreased the growth of control chondrocytes up to 40% compared with those from control sera. We suggest that the pathogenesis of growth failure in CRF may be involved in the increase of circulating IGFBP4 as well as the unidentified small molecular weight uremic serum factors which block the growth of chondrocytes in growth plate.
- Subjects :
- Animals
Cell Proliferation
Cells, Cultured
Chondrocytes metabolism
Insulin-Like Growth Factor Binding Protein 4 analysis
Insulin-Like Growth Factor Binding Protein 4 genetics
Kidney Failure, Chronic metabolism
Kidney Failure, Chronic physiopathology
Liver chemistry
Male
Muscle, Skeletal chemistry
RNA, Messenger analysis
RNA, Messenger metabolism
Rats
Rats, Sprague-Dawley
Somatomedins analysis
Somatomedins metabolism
Chondrocytes cytology
Insulin-Like Growth Factor Binding Protein 4 blood
Kidney Failure, Chronic blood
Subjects
Details
- Language :
- English
- ISSN :
- 1226-3613
- Volume :
- 36
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Experimental & molecular medicine
- Publication Type :
- Academic Journal
- Accession number :
- 15272236
- Full Text :
- https://doi.org/10.1038/emm.2004.33