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Transplanted human fetal neural stem cells survive, migrate, and differentiate in ischemic rat cerebral cortex.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2004 Aug 10; Vol. 101 (32), pp. 11839-44. Date of Electronic Publication: 2004 Jul 27. - Publication Year :
- 2004
-
Abstract
- We characterize the survival, migration, and differentiation of human neurospheres derived from CNS stem cells transplanted into the ischemic cortex of rats 7 days after distal middle cerebral artery occlusion. Transplanted neurospheres survived robustly in naive and ischemic brains 4 wk posttransplant. Survival was influenced by proximity of the graft to the stroke lesion and was negatively correlated with the number of IB4-positive inflammatory cells. Targeted migration of the human cells was seen in ischemic animals, with many human cells migrating long distances ( approximately 1.2 mm) predominantly toward the lesion; in naive rats, cells migrated radially from the injection site in smaller number and over shorter distances (0.2 mm). The majority of migrating cells in ischemic rats had a neuronal phenotype. Migrating cells between the graft and the lesion expressed the neuroblast marker doublecortin, whereas human cells at the lesion border expressed the immature neuronal marker beta-tubulin, although a small percentage of cells at the lesion border also expressed glial fibrillary acid protein (GFAP). Thus, transplanted human CNS (hCNS)-derived neurospheres survived robustly in naive and ischemic brains, and the microenvironment influenced their migration and fate.
- Subjects :
- Animals
Arterial Occlusive Diseases therapy
Biomarkers analysis
Brain Ischemia pathology
Cell Differentiation
Cell Survival
Cerebral Cortex pathology
Doublecortin Protein
Fetus cytology
Humans
Rats
Transplantation, Heterologous
Brain Ischemia therapy
Cell Movement
Neurons cytology
Stem Cell Transplantation
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 101
- Issue :
- 32
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 15280535
- Full Text :
- https://doi.org/10.1073/pnas.0404474101