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[Studies on the characteristic of interferon-gamma mediating resistance in mice infected with Schistosoma japonicum].
- Source :
-
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases [Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi] 2004 Feb 28; Vol. 22 (1), pp. 29-32. - Publication Year :
- 2004
-
Abstract
- Objective: To investigate the molecular characteristic of interferon-gamma mediating protective immunity against schistosomiasis japonica in mice.<br />Methods: CD4+ T cells were isolated from spleens of mice infected with Schistosoma japonicum at different time-points. The cDNA microarray technique combined with RT-PCR was used to explore IFN-gamma inducible GTPase family gene expression profile of CD4+ T cell. IGTP, a representative IFN-gamma, inducible GTPase having vital anti-infection activity, was amplified from spleen of BALB/c mice using RT-PCR, then cloned into pGEM(r)-T easy vector for sequencing.<br />Results: IFN-gamma inducible GTPase family had the similar characteristic over the course of S. japonicum infection. The gene expression of these members were up-regulated or had little change at 3 wk post-infection, then down-modulated from 6 wk to 13 wk post-infection, which was also confirmed by RT-PCR. As for IGTP, two inserts were identified after sequencing. One was 142 bp shorter than another, but the fragment was lost due to low annealing temperature.<br />Conclusion: There is a dramatic inhibition of IFN-gamma pathway and IFN-gamma-dependent anti-infective immunity during the infection of S. japonicum.
- Subjects :
- Animals
CD4-Positive T-Lymphocytes metabolism
Female
GTP Phosphohydrolases genetics
Gene Expression
Gene Expression Profiling
Mice
Mice, Inbred BALB C
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Schistosomiasis japonica metabolism
Up-Regulation
CD4-Positive T-Lymphocytes immunology
GTP Phosphohydrolases metabolism
Interferon-gamma immunology
Schistosoma japonicum immunology
Schistosomiasis japonica immunology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1000-7423
- Volume :
- 22
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases
- Publication Type :
- Academic Journal
- Accession number :
- 15283262