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Validation of an ocular microdialysis technique in rabbits with permanently implanted vitreous probes: systemic and intravitreal pharmacokinetics of fluorescein.
- Source :
-
International journal of pharmaceutics [Int J Pharm] 2004 Aug 20; Vol. 281 (1-2), pp. 79-88. - Publication Year :
- 2004
-
Abstract
- The purpose of this work is to validate a novel ocular microdialysis sampling technique in rabbits with permanently implanted vitreous probes. This objective is achieved by studying the vitreous pharmacokinetics of fluorescein following systemic and intravitreal administration. The rabbits were divided into two groups (groups I and II) based on whether or not they were allowed a recovery period following surgical implantation of probes. The integrity of the blood-retinal barrier was determined by the vitreal protein concentrations and the fluorescein permeability index. Vitreal protein concentrations returned to baseline 48 h after probe implantation and therefore experiments were conducted 72 h post-implantation of probes in rabbits where recovery period was allowed. The permeability indices for fluorescein after systemic administration in group I (without recovery period) and group II (with recovery period) indicated that the integrity of the blood-retinal barrier was maintained and were found out to be 0.55 +/- 0.27 and 0.71 +/- 0.38%, respectively, for the vitreous chamber. Following microdialysis probe implantation in the group II rabbits, the blood-retinal barrier integrity was not compromised. A novel microdialysis technique in rabbits with permanently implanted probes for studying the pharmacokinetics of posterior segment has been developed and characterized.
- Subjects :
- Animals
Blood-Retinal Barrier drug effects
Blood-Retinal Barrier metabolism
Drug Evaluation, Preclinical methods
Eye drug effects
Eye metabolism
Fluorescein administration & dosage
Half-Life
Injections methods
Male
Models, Animal
Postoperative Care methods
Rabbits
Time Factors
Vitreous Body chemistry
Vitreous Body metabolism
Catheters, Indwelling trends
Fluorescein pharmacokinetics
Microdialysis methods
Vitreous Body drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0378-5173
- Volume :
- 281
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- International journal of pharmaceutics
- Publication Type :
- Academic Journal
- Accession number :
- 15288345
- Full Text :
- https://doi.org/10.1016/j.ijpharm.2004.05.028