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Immune regulatory effect of pHSP65 DNA therapy in pulmonary tuberculosis: activation of CD8+ cells, interferon-gamma recovery and reduction of lung injury.
- Source :
-
Immunology [Immunology] 2004 Sep; Vol. 113 (1), pp. 130-8. - Publication Year :
- 2004
-
Abstract
- A DNA vaccine based on the heat-shock protein 65 Mycobacterium leprae gene (pHSP65) presented a prophylactic and therapeutic effect in an experimental model of tuberculosis. In this paper, we addressed the question of which protective mechanisms are activated in Mycobacterium tuberculosis-infected mice after immune therapy with pHSP65. We evaluated activation of the cellular immune response in the lungs of infected mice 30 days after infection (initiation of immune therapy) and in those of uninfected mice. After 70 days (end of immune therapy), the immune responses of infected untreated mice, infected pHSP65-treated mice and infected pCDNA3-treated mice were also evaluated. Our results show that the most significant effect of pHSP65 was the stimulation of CD8+ lung cell activation, interferon-gamma recovery and reduction of lung injury. There was also partial restoration of the production of tumour necrosis factor-alpha. Treatment with pcDNA3 vector also induced an immune stimulatory effect. However, only infected pHSP65-treated mice were able to produce significant levels of interferon-gamma and to restrict the growth of bacilli.
- Subjects :
- Animals
CD18 Antigens metabolism
CD28 Antigens metabolism
CD4-Positive T-Lymphocytes immunology
Chaperonin 60
Fas Ligand Protein
Female
Lymphocyte Activation immunology
Membrane Glycoproteins metabolism
Mice
Mice, Inbred BALB C
Tuberculosis, Pulmonary immunology
Tuberculosis, Pulmonary microbiology
Tuberculosis, Pulmonary pathology
Up-Regulation
fas Receptor metabolism
Bacterial Proteins genetics
CD8-Positive T-Lymphocytes immunology
Chaperonins genetics
Interferon-gamma biosynthesis
Tuberculosis, Pulmonary therapy
Vaccines, DNA therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 0019-2805
- Volume :
- 113
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 15312144
- Full Text :
- https://doi.org/10.1111/j.1365-2567.2004.01931.x