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Vaccine-induced immunity against Helicobacter pylori infection is impaired in IL-18-deficient mice.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2004 Sep 01; Vol. 173 (5), pp. 3348-56. - Publication Year :
- 2004
-
Abstract
- Protective immunity against Helicobacter pylori infection in mice has been associated with a strong Th1 response, involving IL-12 as well as IFN-gamma, but recent studies have also demonstrated prominent eosinophilic infiltration, possibly linked to local Th2 activity in the gastric mucosa. In this study we investigated the role of IL-18, because this cytokine has been found to be a coregulator of Th1 development as well as involved in Th2-type responses with local eotaxin production that could influence gastric eosinophilia and resistance to infection. We found that IL-18(-/-) mice failed to develop protection after oral immunization with H. pylori lysate and cholera toxin adjuvant, indicating an important role of IL-18 in protection. Well-protected C57BL/6 wild-type (WT) mice demonstrated substantial influx of CD4(+) T cells and eosinophilic cells in the gastric mucosa, whereas IL-18(-/-) mice had less gastritis, few CD4(+) T cells, and significantly reduced numbers of eosinophilic cells. T cells in well-protected WT mice produced increased levels of IFN-gamma and IL-18 to recall Ag. By contrast, unprotected IL-18(-/-) mice exhibited significantly reduced gastric IFN-gamma and specific IgG2a Ab levels. Despite differences in gastric eosinophilic cell infiltration, protected WT and unprotected IL-18(-/-) mice had comparable levels of local eotaxin, suggesting that IL-18 influences protection via Th1 development and IFN-gamma production rather than through promoting local production of eotaxin and eosinophilic cell infiltration.
- Subjects :
- Animals
Antigens, Bacterial immunology
Chemokine CCL11
Chemokines, CC metabolism
Gastritis immunology
Gastritis metabolism
Helicobacter Infections immunology
Interferon-gamma metabolism
Interleukin-18 genetics
Interleukin-18 immunology
Interleukin-18 metabolism
Mice
Helicobacter Infections prevention & control
Helicobacter pylori immunology
Interleukin-18 deficiency
Vaccines immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 173
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 15322198
- Full Text :
- https://doi.org/10.4049/jimmunol.173.5.3348