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cDNA microarray analysis of early gene expression profiles associated with hepatitis B virus X protein-mediated hepatocarcinogenesis.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2004 Sep 24; Vol. 322 (3), pp. 827-35. - Publication Year :
- 2004
-
Abstract
- Chronic hepatitis B virus (HBV) infection is one of the major causes of hepatocellular carcinoma. HBV encodes an oncogenic hepatitis B virus X protein (HBx), which can transactivate host cell transcriptional machinery and mediate cellular transformation. To disclose the early genetic response in HBx-mediated transformation process, we constructed a conditional HBx-expressing hepatocyte cell line, which allows us to compare the gene expression profiles under controllable HBx induction. A cDNA microarray containing more than 8700 mouse genes and ESTs was utilized to examine the gene expression profiles. We identified 260 candidate genes and 259 ESTs which have shown aberrant expression under HBx induction. Most of them are involved in signal transduction pathway, cell cycle control, metastasis, transcriptional regulation, immune response, and metabolism. These results provide additional insight into early cellular targets of HBx, which could give us a better understanding of the function of HBx and their progressive changes during HBx-mediated hepatocarcinogenesis.
- Subjects :
- Base Sequence
Carcinoma, Hepatocellular virology
Cell Division
Cell Line
Cell Transformation, Neoplastic
Cloning, Molecular
DNA Primers
Hepatitis B Antigens physiology
Humans
Liver Neoplasms virology
Recombinant Proteins metabolism
Trans-Activators genetics
Transcriptional Activation
Transfection
Viral Regulatory and Accessory Proteins
Carcinoma, Hepatocellular genetics
DNA, Complementary genetics
Gene Expression Profiling methods
Liver Neoplasms genetics
Oligonucleotide Array Sequence Analysis methods
Trans-Activators physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 322
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 15336538
- Full Text :
- https://doi.org/10.1016/j.bbrc.2004.07.188