Back to Search
Start Over
CC chemokine ligands 25 and 28 play essential roles in intestinal extravasation of IgA antibody-secreting cells.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2004 Sep 15; Vol. 173 (6), pp. 3668-75. - Publication Year :
- 2004
-
Abstract
- CCL25 (also known as thymus-expressed chemokine) and CCL28 (also known as mucosae-associated epithelial chemokine) play important roles in mucosal immunity by recruiting IgA Ab-secreting cells (ASCs) into mucosal lamina propria. However, their exact roles in vivo still remain to be defined. In this study, we first demonstrated in mice that IgA ASCs in small intestine expressed CCR9, CCR10, and CXCR4 on the cell surface and migrated to their respective ligands CCL25, CCL28, and CXCL12 (also known as stromal cell-derived factor 1), whereas IgA ASCs in colon mainly expressed CCR10 and CXCR4 and migrated to CCL28 and CXCL12. Reciprocally, the epithelial cells of small intestine were immunologically positive for CCL25 and CCL28, whereas those of colon were positive for CCL28 and CXCL12. Furthermore, the venular endothelial cells in small intestine were positive for CCL25 and CCL28, whereas those in colon were positive for CCL28, suggesting their direct roles in extravasation of IgA ASCs. Consistently, in mice orally immunized with cholera toxin (CT), anti-CCL25 suppressed homing of CT-specific IgA ASCs into small intestine, whereas anti-CCL28 suppressed homing of CT-specific IgA ASCs into both small intestine and colon. Reciprocally, CT-specific ASCs and IgA titers in the blood were increased in mice treated with anti-CCL25 or anti-CCL28. Anti-CXCL12 had no such effects. Finally, both CCL25 and CCL28 were capable of enhancing alpha4 integrin-dependent adhesion of IgA ASCs to mucosal addressin cell adhesion molecule-1 and VCAM-1. Collectively, CCL25 and CCL28 play essential roles in intestinal homing of IgA ASCs primarily by mediating their extravasation into intestinal lamina propria.<br /> (Copyright 2004 The American Association of Immunologists, Inc.)
- Subjects :
- Animals
Antibody-Producing Cells metabolism
Cell Adhesion immunology
Cell Adhesion Molecules
Cell Membrane immunology
Cell Membrane metabolism
Chemokine CCL27
Chemokine CXCL12
Chemokines biosynthesis
Chemokines immunology
Chemokines, CC biosynthesis
Chemokines, CC immunology
Chemokines, CXC biosynthesis
Chemokines, CXC immunology
Cholera Toxin administration & dosage
Cholera Toxin immunology
Female
Humans
Immune Sera administration & dosage
Immunoglobulins metabolism
Immunohistochemistry
Integrin alpha4 physiology
Intestinal Mucosa cytology
Mice
Mice, Inbred BALB C
Mucoproteins metabolism
Organ Specificity immunology
Receptors, CCR
Receptors, CCR10
Receptors, CXCR4 biosynthesis
Receptors, Chemokine biosynthesis
Staining and Labeling
Vascular Cell Adhesion Molecule-1 metabolism
Antibody-Producing Cells cytology
Antibody-Producing Cells immunology
Chemokines physiology
Chemokines, CC physiology
Chemotaxis, Leukocyte immunology
Immunoglobulin A biosynthesis
Intestinal Mucosa immunology
Intestinal Mucosa metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 173
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 15356112
- Full Text :
- https://doi.org/10.4049/jimmunol.173.6.3668