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Independent inheritance of genes regulating two subpopulations of mouse clonogenic keratinocyte stem cells.
- Source :
-
The journal of investigative dermatology. Symposium proceedings [J Investig Dermatol Symp Proc] 2004 Sep; Vol. 9 (3), pp. 253-60. - Publication Year :
- 2004
-
Abstract
- Mouse keratinocyte stem cells originate from the bulge of hair follicle, and, according to definition, possess a clonogenic activity in vitro. We have investigated seven inbred (C57BL/6, C3H, DBA/2, BALB/c, FVB) and outbred (SENCAR, CD-1) mouse strains and found that three genetically distinct subsets of mouse strains differ significantly in the frequency of clonogenic activity in vitro. The analysis of keratinocyte colonies in two reciprocal backcross [C57BL/6 x (BALB/c x C57BL/6); BALB/c x (BALB/c x C57BL/6)] and intercross [(BALB/c x C57BL/ 6)F2] of BALB/c and C57BL/6 mice allowed us to identify two subpopulations of clonogenic keratinocytes able to produce small (less than 2 mm2) and large (more than 2 mm2) colonies. We conducted linkage analysis and found that small colonies associated with mouse chromosomes 1, 6, 7, 8, and 9; but large colonies--with the chromosome 4. We defined locus on the chromosome 9 that associated with small colonies as keratinocyte stem cell locus 1 (Ksc1), and locus on the mouse chromosome 4 associated with large colonies-keratinocyte stem cell locus 2 (Ksc2). Ksc1 and loci on chromosomes 6 and 7 are close if not equal to loci associated with sensitivity to skin carcinogenesis. We conclude that two subpopulations of stem cells able to produce small and large colonies regulated by different genes and genes regulating small colonies might be responsible for sensitivity to skin carcinogenesis.
- Subjects :
- Animals
Cell Division genetics
Clone Cells
Female
Genetic Markers
Genotype
Lod Score
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred DBA
Mice, Inbred SENCAR
Phenotype
Keratinocytes cytology
Keratinocytes physiology
Skin Physiological Phenomena genetics
Stem Cells cytology
Stem Cells physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1087-0024
- Volume :
- 9
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The journal of investigative dermatology. Symposium proceedings
- Publication Type :
- Academic Journal
- Accession number :
- 15369221
- Full Text :
- https://doi.org/10.1111/j.1087-0024.2004.09307.x