Back to Search
Start Over
Catheter-mediated delivery of adenoviral vectors expressing beta-adrenergic receptor kinase C-terminus inhibits intimal hyperplasia and luminal stenosis in rabbit iliac arteries.
- Source :
-
The journal of gene medicine [J Gene Med] 2004 Oct; Vol. 6 (10), pp. 1061-8. - Publication Year :
- 2004
-
Abstract
- Background: Previous studies have shown that incubation of balloon-injured rat carotid arteries with adenoviral vectors encoding the carboxyl terminus of the beta-adrenergic receptor kinase (Ad2/betaARKct) for 30 min reduces neointima formation. However, it is unclear whether this beneficial effect of betaARKct could be achieved using a catheter-based vector delivery system and whether the observed inhibition of neointima formation translated into a reduction of vessel stenosis.<br />Methods: In this study, Ad2/betaARKct was infused into the balloon-injured site of rabbit iliac arteries using a porous infusion catheter over 2 min. Twenty-eight days after gene transfer, angiographic and histological assessments were performed.<br />Results: Angiographic and histological assessments indicate significant (p < 0.05) inhibition of iliac artery neointima formation and lumen stenosis by Ad2/betaARKct. Our studies demonstrate that an inhibitory effect of Ad2/betaARKct on neointima formation is achievable using a catheter-based vector delivery system and that the inhibition of neointima formation translates into a gain in the vessel minimal luminal diameter. The extent of inhibition (35%) was comparable to that observed with adenoviral-mediated expression of thymidine kinase plus ganciclovir treatment, a cytotoxic gene therapy approach for restenosis.<br />Conclusions: These results suggest that adenoviral-mediated gene transfer of betaARKct is a clinically viable cytostatic gene therapy strategy for the treatment of restenosis.<br /> (Copyright (c) 2004 John Wiley & Sons, Ltd.)
- Subjects :
- Angiography
Animals
Carotid Arteries pathology
Constriction, Pathologic prevention & control
DNA Primers chemistry
Hyperplasia pathology
Protein Structure, Tertiary
Rabbits
Time Factors
beta-Adrenergic Receptor Kinases
beta-Galactosidase metabolism
Adenoviridae genetics
Constriction, Pathologic therapy
Cyclic AMP-Dependent Protein Kinases chemistry
Cyclic AMP-Dependent Protein Kinases genetics
Gene Transfer Techniques
Genetic Therapy methods
Genetic Vectors
Hyperplasia therapy
Iliac Artery pathology
Tunica Intima pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1099-498X
- Volume :
- 6
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- The journal of gene medicine
- Publication Type :
- Academic Journal
- Accession number :
- 15386742
- Full Text :
- https://doi.org/10.1002/jgm.592