Back to Search
Start Over
Safety testing of infracyanine green using retinal pigment epithelium and glial cell cultures.
- Source :
-
Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2004 Oct; Vol. 45 (10), pp. 3697-703. - Publication Year :
- 2004
-
Abstract
- Purpose: To undertake safety testing of infracyanine green (IFCG) in a cell culture model.<br />Methods: Experiments were undertaken in a cell culture model used previously to perform safety testing of indocyanine green (ICG). Human retinal pigment epithelium (RPE) and Müller cells were exposed to IFCG for 5 minutes, over a range of concentrations up to 0.5%. Experiments were repeated, using double-staining with trypan blue. Cell viability was measured at days 1, 5, and 15 using a mitochondrial dehydrogenase assay and a fluorescent live-dead probe containing calcein and ethidium homodimer-1. Viability was measured after exposure to 0.5% IFCG and 5 minutes of illumination with a vitrectomy endolight powered by a xenon light source.<br />Results: RPE viability was not reduced over the range of concentrations and follow-up intervals. RPE cells exposed to IFCG and illumination had reduced viability relative to the negative control (cells exposed to saline), but not relative to those exposed to saline and illumination. Glial cells showed reduced viability at days 1 and 5, but not day 15. Illumination did not further reduce viability.<br />Conclusions: IFCG has been advocated as a safer macular vital stain than ICG. These results suggest that it is less likely to produce phototoxicity, but despite being nearly iso-osmolar, IFCG also produces damage in cultured glial cells.
- Subjects :
- Cell Culture Techniques
Cell Survival drug effects
Cell Survival radiation effects
Ethidium analogs & derivatives
Humans
Neuroglia pathology
Neuroglia radiation effects
Pigment Epithelium of Eye pathology
Pigment Epithelium of Eye radiation effects
Safety
Trypan Blue
Coloring Agents toxicity
Indocyanine Green analogs & derivatives
Indocyanine Green toxicity
Neuroglia drug effects
Pigment Epithelium of Eye drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0146-0404
- Volume :
- 45
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Investigative ophthalmology & visual science
- Publication Type :
- Academic Journal
- Accession number :
- 15452079
- Full Text :
- https://doi.org/10.1167/iovs.04-0387