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In vivo labeling and analysis of epidermal stem cells.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2005; Vol. 289, pp. 73-8. - Publication Year :
- 2005
-
Abstract
- To identify slowly cycling (label-retaining) keratinocytes in the cutaneous epithelium of mice, newborn female pups were injected with [3H]-thymidine twice daily for 3 d beginning the third day after birth. At 8-10 wk of age, skin from the dorsal side of the mice was removed and fixed. The skin was processed for paraffin embedding, and serial sections were prepared. Dewaxed and ethanol-treated slides were dried and dipped into autoradiographic emulsion and allowed to dry in light tight box for 4 wk at 4 degrees C. After fixation with photographic fixer, the slides were washed, lightly stained, and viewed with a light microscope to observe and quantify cells that retained the labeling. Label-retaining cells were identified by the black silver grains over their nuclei.
Details
- Language :
- English
- ISSN :
- 1064-3745
- Volume :
- 289
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 15502171
- Full Text :
- https://doi.org/10.1385/1-59259-830-7:073