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Immunostimulating properties of intragastrically administered Acetobacter-derived soluble branched (1,4)-beta-D-glucans decrease murine susceptibility to Listeria monocytogenes.
- Source :
-
Infection and immunity [Infect Immun] 2004 Dec; Vol. 72 (12), pp. 7005-11. - Publication Year :
- 2004
-
Abstract
- We previously found that AC-1, an extracellular polysaccharide, produced by Acetobacter xylinum and composed of (1,4)-beta-D-glucan with branches of glucosyl residues, showed a strong activity to induce production of interleukin-12 (IL-12) p40 and tumor necrosis factor alpha by macrophages in vitro via Toll-like receptor 4 (TLR-4) signaling. In the present study, we examined the effect of oral administration of AC-1 on protective immunity against Listeria monocytogenes. Mice were given AC-1 or phosphate-buffered saline (PBS) intragastrically 2 days before, on the day of, and 2 days after an intraperitoneal inoculation of L. monocytogenes. The survival rate of AC-1-treated mice was significantly improved and bacterial growth in AC-1-treated mice was severely retarded compared to those of PBS-treated mice after infection with L. monocytogenes. IL-12 p40 levels in serum and magnitudes of CD4+ Th1 and CD8+ Tc1 responses against Listeria antigen were significantly higher in AC-1-treated mice than in PBS-treated mice. The effect of AC-1 on antilisterial activity was diminished in C3H/HeJ mice carrying mutated TLR-4. Thus, AC-1, a potent IL-12 inducer through TLR-4, enhanced protective immunity against L. monocytogenes via augmentation of Th1 responses. These results suggest that infectious processes driven by intracellular microorganisms could be prevented to develop by the (1,4)-beta-D-glucan.
- Subjects :
- Animals
Disease Susceptibility
Interferon-gamma biosynthesis
Interleukin-12 biosynthesis
Interleukin-12 Subunit p40
Mice
Mice, Inbred C3H
Mice, Inbred C57BL
Protein Subunits biosynthesis
Receptors, Cell Surface physiology
Toll-Like Receptor 4
Tumor Necrosis Factor-alpha biosynthesis
beta-Glucans administration & dosage
Acetobacter chemistry
Adjuvants, Immunologic pharmacology
Listeria monocytogenes immunology
Listeriosis immunology
beta-Glucans pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0019-9567
- Volume :
- 72
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 15557623
- Full Text :
- https://doi.org/10.1128/IAI.72.12.7005-7011.2004