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[Effects of genistein on proliferation and differentiation of cultured osteoblasts in vitro].
- Source :
-
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials [Zhong Yao Cai] 2002 Nov; Vol. 25 (11), pp. 803-5. - Publication Year :
- 2002
-
Abstract
- Objective: To study the effects of genistein on proliferation and differentiation of cultured osteoblasts in vitro.<br />Methods: MTT and PNPP were used separately to observe the proliferation and the activity of ALP of osteoblasts cultured in vitro with different concentration genistein for different incubation periods. Protein was measured by Lowry method.<br />Results: It was found that genistein had the effects on promoting proliferation of osteoblasts cultured for 24 h and 48 h at concentration ranging from 1 x 10(-9) mol/L to 1 x 0(-5) mol/L. The activity of ALP was stimulated by genistein at concentration ranging from 1 x 10(-7) mol/L to 1 x 10(-5) mol/L after 72 h incubation.<br />Conclusion: Genistein has the effects on stimulating the proliferation and differentiation of cultured osteoblasts in vitro.
- Subjects :
- Alkaline Phosphatase metabolism
Animals
Cells, Cultured
Female
Isoflavones chemistry
Male
Osteoblasts cytology
Rats
Rats, Sprague-Dawley
Glycine max chemistry
Cell Differentiation drug effects
Cell Proliferation drug effects
Drugs, Chinese Herbal pharmacology
Genistein pharmacology
Osteoblasts drug effects
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1001-4454
- Volume :
- 25
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
- Publication Type :
- Academic Journal
- Accession number :
- 15562707