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Normal platelets possess the soluble form of IL-6 receptor.
- Source :
-
Cytokine [Cytokine] 2005 Jan 07; Vol. 29 (1), pp. 13-7. - Publication Year :
- 2005
-
Abstract
- Interleukin 6 is a multifunctional cytokine that exerts its biological activity through binding to an 80 Kd specific receptor (IL-6Ralpha) and a 130 Kd signal-transducing unit (gp130). A 55 Kd soluble IL-6R (IL-6sR) has also been described which, after binding to IL-6 is also able to activate gp130. The presence of IL-6Ralpha was described in some megakaryoblastic cell lines but is still controversial in normal megakaryocytes. In this study we demonstrate the presence of intraplatelet IL-6sR by Western blot through the appearance of a 55 Kd protein and the finding of detectable amounts of IL-6sR in the platelet content by ELISA technique. Besides, we showed IL-6sR release during platelet activation induced by thrombin and a complex of ADP and epinephrine. IL-6Ralpha on platelet membrane could not be found neither by Western blot nor by flow cytometry. The IL-6sR released during platelet activation and complexed to IL-6 could act on cell types such as endothelial cells that do not possess IL-6Ralpha through binding to gp130.Besides, since we could not find IL-6R on platelet membrane, the potentiating effect of IL-6 on platelet function could be explained through binding of IL-6sR/IL-6 complex to platelet membrane gp130.
- Subjects :
- Blotting, Western
Contactins
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Humans
Immunoblotting
Neural Cell Adhesion Molecules metabolism
Platelet Activation
Protein Binding
Thrombin metabolism
Blood Platelets metabolism
Receptors, Interleukin-6 biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1043-4666
- Volume :
- 29
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Cytokine
- Publication Type :
- Academic Journal
- Accession number :
- 15579373
- Full Text :
- https://doi.org/10.1016/j.cyto.2004.09.003