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Expression of the KIT protein (CD117) in primary cutaneous mast cell tumors of the dog.
- Source :
-
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc [J Vet Diagn Invest] 2004 Nov; Vol. 16 (6), pp. 554-61. - Publication Year :
- 2004
-
Abstract
- Thirty-one canine cutaneous masses, diagnosed as mast cell tumors (MCT) by histopathologic analysis, were used to evaluate the immunohistochemical pattern of expression of KIT protein (CD117), a type III tyrosine kinase protein involved in mast cell growth and differentiation. Lesions were graded as I (well differentiated), II (intermediate differentiation), or III (poorly differentiated) according to the following morphologic features: invasiveness, cellularity and cellular morphology, mitotic index, and stromal reaction. Immunohistochemical KIT expression was compared with histologic grade and some histomorphologic features (cell differentiation and nuclear grade) evaluated separately. A possible predictive role of biologic behavior in MCTs for KIT expression was also investigated. Immunohistochemical analysis revealed three different patterns of KIT expression: a cytoplasmic diffuse pattern, a membranous pattern with immunostaining located on the cell surface, and a cytoplasmic perinuclear pattern, where KIT expression was detected in the cytoplasm of the neoplastic mast cells, close to the nucleus. Statistical analysis showed a close relationship between different KIT immunohistochemical patterns and histologic grade (P < 0.00000), cell differentiation (P < 0.00000), and nuclear grade (P < 0.0024). According to Kaplan-Meier-estimated survival curves compared by survival analysis, KIT expression was significantly associated with survival time (P = 0.037) but not cancer-free interval (P = 0.50). Similar to other well-known histomorphological features, KIT expression is a useful parameter of malignancy in cutaneous MCTs. KIT expression also predicted the biological behavior of the tumors in this study.
- Subjects :
- Animals
Cell Differentiation
Dog Diseases pathology
Dogs
Female
Immunohistochemistry veterinary
Male
Mast-Cell Sarcoma metabolism
Mast-Cell Sarcoma pathology
Neoplasm Invasiveness
Skin Neoplasms metabolism
Skin Neoplasms pathology
Dog Diseases metabolism
Gene Expression genetics
Mast-Cell Sarcoma veterinary
Proto-Oncogene Proteins c-kit metabolism
Skin Neoplasms veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1040-6387
- Volume :
- 16
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
- Publication Type :
- Academic Journal
- Accession number :
- 15586571
- Full Text :
- https://doi.org/10.1177/104063870401600610