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FK506 suppresses E-selectin, ICAM-1 and VCAM-1 expression on vascular endothelial cells by inhibiting tumor necrosis factor alpha secretion from peripheral blood mononuclear cells.
- Source :
-
Cytokine [Cytokine] 2005 Jan 21; Vol. 29 (2), pp. 67-71. - Publication Year :
- 2005
-
Abstract
- FK506 suppresses activation of T cells; however, it down-regulates E-selectin, ICAM-1 and VCAM-1 expression in inflamed tissues. In this study, we investigated the effect of FK506 on expression of those adhesion molecules on human vascular endothelial cells (HMVEC). Culture supernatant from peripheral blood mononuclear cells (PBMC) stimulated with anti-CD3 plus anti-CD2 antibodies effectively induced the expression of E-selectin, ICAM-1 and VCAM-1 on HMVEC, and treatment with FK506 down-regulated their expression. Culture supernatant contained tumor necrosis factor (TNF) alpha and interleukin (IL)-1beta, which effectively induced adhesion molecules, and FK506 suppressed both cytokine secretions. TNFalpha content in culture supernatant was parallel to the induction of adhesion molecules by the culture supernatant. IL-1beta content was not enough to induce those adhesion molecules. Anti-TNFalpha antibody completely inhibited those expressions. FK506 did not inhibit either TNFalpha- or IL-1beta-induced expression of adhesion molecules, or viability of HMVEC. These results indicate that FK506 suppresses migration of inflammatory cells through the inhibition of TNFalpha secretion from leukocytes.
- Subjects :
- Endothelium, Vascular cytology
Endothelium, Vascular metabolism
Enzyme-Linked Immunosorbent Assay
Humans
Interleukin-1 metabolism
Interleukin-1 physiology
Monocytes metabolism
Tumor Necrosis Factor-alpha metabolism
E-Selectin drug effects
Endothelium, Vascular drug effects
Intercellular Adhesion Molecule-1 drug effects
Monocytes drug effects
Tacrolimus pharmacology
Tumor Necrosis Factor-alpha antagonists & inhibitors
Vascular Cell Adhesion Molecule-1 drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1043-4666
- Volume :
- 29
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Cytokine
- Publication Type :
- Academic Journal
- Accession number :
- 15598440
- Full Text :
- https://doi.org/10.1016/j.cyto.2004.04.006