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Menadione metabolism to thiodione in hepatoblastoma by scanning electrochemical microscopy.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2004 Dec 21; Vol. 101 (51), pp. 17582-7. Date of Electronic Publication: 2004 Dec 15. - Publication Year :
- 2004
-
Abstract
- The cytotoxicity of menadione on hepatocytes was studied by using the substrate generation/tip collection mode of scanning electrochemical microscopy by exposing the cells to menadione and detecting the menadione-S-glutathione conjugate (thiodione) that is formed during the cellular detoxication process and is exported from the cell by an ATP-dependent pump. This efflux was electrochemically detected and allowed scanning electrochemical microscopy monitoring and imaging of single cells and groups of highly confluent live cells. Based on a constant flux model, approximately 6 x 10(6) molecules of thiodione per cell per second are exported from monolayer cultures of Hep G2 cells.
- Subjects :
- Cell Line, Tumor
Electrochemistry
Glutathione chemistry
Hepatoblastoma pathology
Humans
Inactivation, Metabolic
Liver drug effects
Liver metabolism
Liver pathology
Molecular Structure
Time Factors
Vitamin K 3 chemistry
Vitamin K 3 toxicity
Glutathione analogs & derivatives
Glutathione metabolism
Hepatoblastoma metabolism
Microscopy methods
Vitamin K 3 analogs & derivatives
Vitamin K 3 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 101
- Issue :
- 51
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 15601769
- Full Text :
- https://doi.org/10.1073/pnas.0407613101