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n-3 fatty acids modulate T-cell calcium signaling in obese macrosomic rats.
- Source :
-
Obesity research [Obes Res] 2004 Nov; Vol. 12 (11), pp. 1744-53. - Publication Year :
- 2004
-
Abstract
- Objective: We investigated the effects of a diet containing EPAX-7010, rich in PUFAs such as eicosapentaenoic acid [20:5(n-3)] and docosahexaenoic acid [22:6(n-3)], i.e., a PUFA/EPAX regimen, on T-cell activation in diabetic pregnant rats and their obese pups.<br />Research Methods and Procedures: Mild hyperglycemia in pregnant rats was induced by intraperitoneal injection of streptozotocin on Day 5 of gestation. T-cell blastogenesis was assayed by using (3)H-thymidine, whereas intracellular free calcium concentrations ([Ca(2+)]i) were measured by using Fura-2 in diabetic pregnant rats and their obese offspring.<br />Results: Concavalin-A-stimulated T-cell proliferation was decreased in both pregnant diabetic rats and their obese pups as compared with control animals. Feeding the PUFA/EPAX diet restored T-cell proliferation in both groups of animals. We also employed ionomycin, which at 50 nM opens calcium channels, and thapsigargin (TG), which recruits [Ca(2+)]i from endoplasmic reticulum pool. We observed that ionomycin-induced increases in [Ca(2+)]i in T-cells of diabetic mothers and obese offspring were greater than in those of control rats. Furthermore, feeding PUFA/EPAX diet diminished significantly the ionomycin-evoked rise in [Ca(2+)]i in diabetic and obese animals. TG-induced increases in [Ca(2+)]i in T-cells of diabetic pregnant rats and their obese offspring were greater than in those of control rats. The feeding of the experimental diet significantly curtailed the TG-evoked increases in [Ca(2+)]i in both diabetic and obese rats.<br />Discussion: Together, these observations provide evidence that T-cell activation and T-cell calcium signaling are altered during gestational diabetes and macrosomia. Hence, dietary fish oils, particularly eicosapentaenoic acid and docosahexaenoic acid, may restore these T-cell abnormalities.
- Subjects :
- Animals
Concanavalin A pharmacology
Diabetes Mellitus, Experimental immunology
Dietary Fats, Unsaturated administration & dosage
Female
Fetal Macrosomia etiology
Gestational Age
Ionomycin pharmacology
Lymphocyte Activation drug effects
Obesity etiology
Pregnancy
Pregnancy in Diabetics complications
Rats
Rats, Wistar
Signal Transduction drug effects
Spleen
T-Lymphocytes metabolism
Thapsigargin pharmacology
Calcium metabolism
Fatty Acids, Omega-3 administration & dosage
Fetal Macrosomia immunology
Obesity immunology
Pregnancy in Diabetics immunology
T-Lymphocytes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1071-7323
- Volume :
- 12
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Obesity research
- Publication Type :
- Academic Journal
- Accession number :
- 15601968
- Full Text :
- https://doi.org/10.1038/oby.2004.216