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Dendritic cells and NK cells stimulate bystander T cell activation in response to TLR agonists through secretion of IFN-alpha beta and IFN-gamma.
Dendritic cells and NK cells stimulate bystander T cell activation in response to TLR agonists through secretion of IFN-alpha beta and IFN-gamma.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2005 Jan 15; Vol. 174 (2), pp. 767-76. - Publication Year :
- 2005
-
Abstract
- Recognition of conserved features of infectious agents by innate pathogen receptors plays an important role in initiating the adaptive immune response. We have investigated early changes occurring among T cells after injection of TLR agonists into mice. Widespread, transient phenotypic activation of both naive and memory T cells was observed rapidly after injection of molecules acting through TLR3, -4, -7, and -9, but not TLR2. T cell activation was shown to be mediated by a combination of IFN-alphabeta, secreted by dendritic cells (DCs), and IFN-gamma, secreted by NK cells; notably, IFN-gamma-secreting NK cells expressed CD11c and copurified with DCs. Production of IFN-gamma by NK cells could be stimulated by DCs from TLR agonist-injected mice, and although soluble factors secreted by LPS-stimulated DCs were sufficient to induce IFN-gamma, maximal IFN-gamma production required both direct contact of NK cells with DCs and DC-secreted cytokines. In vitro, IFN-alphabeta, IL-18, and IL-12 all contributed to DC stimulation of NK cell IFN-gamma, whereas IFN-alphabeta was shown to be important for induction of T cell bystander activation and NK cell IFN-gamma production in vivo. The results delineate a pathway involving innate immune mediators through which TLR agonists trigger bystander activation of T cells.
- Subjects :
- Animals
CD11c Antigen biosynthesis
Cell Differentiation immunology
Cells, Cultured
Cytokines metabolism
Cytokines physiology
Dendritic Cells cytology
Immunologic Memory immunology
Immunophenotyping
Interferon Type I biosynthesis
Interferon Type I physiology
Interferon-gamma biosynthesis
Interferon-gamma deficiency
Interferon-gamma genetics
Lipopolysaccharides pharmacology
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Resting Phase, Cell Cycle immunology
T-Lymphocyte Subsets metabolism
Toll-Like Receptor 2
Toll-Like Receptor 3
Toll-Like Receptors
Bystander Effect immunology
Dendritic Cells immunology
Interferon Type I metabolism
Interferon-gamma metabolism
Killer Cells, Natural immunology
Lymphocyte Activation immunology
Membrane Glycoproteins agonists
Receptors, Cell Surface agonists
T-Lymphocyte Subsets immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 174
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 15634897
- Full Text :
- https://doi.org/10.4049/jimmunol.174.2.767