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Apoptosis in chronic viral hepatitis parallels histological activity: an immunohistochemical investigation using anti-activated caspase-3 and M30 cytodeath antibody.
- Source :
-
International journal of experimental pathology [Int J Exp Pathol] 2005 Feb; Vol. 86 (1), pp. 19-24. - Publication Year :
- 2005
-
Abstract
- Apoptosis is implicated as a major pathogenic mechanism in chronic hepatitis B and C. Previous studies of the relationship between apoptotic rates and histological necroinflammatory activity have produced conflicting results. Hepatocyte apoptosis was assessed in liver tissue from 32 cases of chronic viral hepatitis, seven cases of hepatocellular carcinoma (HCC) and six cases of steatohepatitis as non-viral disease controls and eight cases of control liver. Apoptotic rates were measured using H&E morphological assessment and immunohistochemical staining with antibodies to activated caspase-3 and M30. Histological necroinflammatory activity of viral hepatitis cases was scored using the Knodell scoring system, and the cases were divided according to their score into group 1 (mean 2.43 +/- 0.48) and group 2 (mean 7.80 +/- 0.49). Apoptotic indices were significantly higher in group 2 than group 1 using H&E (11.53 +/- 2.70 vs. 0 +/- 0, P=0.015) and activated caspase-3 (22.01 +/- 5.27 vs. 1.79 +/- 1.79, P=0.03) methods but were not significantly higher with M30 (3.80 +/- 1.74 vs. 0 +/- 0, P=0.207). Apoptotic scores using an antibody to activated caspase-3 are significantly higher in cases of chronic viral hepatitis with greater histological necroinflammatory scores, supporting a central role for apoptosis in disease pathogenesis. This method offers an alternative to routine histological assessment for measuring disease activity.
- Subjects :
- Antibodies, Monoclonal immunology
Carcinoma, Hepatocellular pathology
Caspase 3
Caspases immunology
Caspases metabolism
Fatty Liver pathology
Hepatitis B, Chronic metabolism
Hepatitis C, Chronic metabolism
Humans
Immunoenzyme Techniques
Keratins immunology
Keratins metabolism
Liver Neoplasms pathology
Retrospective Studies
Apoptosis
Hepatitis B, Chronic pathology
Hepatitis C, Chronic pathology
Subjects
Details
- Language :
- English
- ISSN :
- 0959-9673
- Volume :
- 86
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- International journal of experimental pathology
- Publication Type :
- Academic Journal
- Accession number :
- 15676029
- Full Text :
- https://doi.org/10.1111/j.0959-9673.2005.00411.x