Back to Search
Start Over
Oxytocin microinjected into dorsal motor nucleus of the vagus excites gallbladder motility via NMDA receptor-NO-cGMP pathway.
- Source :
-
Brain research [Brain Res] 2005 Jan 25; Vol. 1032 (1-2), pp. 116-22. - Publication Year :
- 2005
-
Abstract
- Our recent study indicated that, in the dorsal motor nucleus of the vagus (DMV), the N-methyl-D-aspartic acid (NMDA) receptor-nitric oxide (NO)-cGMP pathway participated in the regulation of gallbladder motility in rabbits. Oxytocin (OT) is involved as a neurotransmitter in autonomic regulation. The aim of the present experiments is to investigate the effect of OT microinjected into DMV on the gallbladder motility and the involvement of NMDA receptor-NO-cGMP pathway. A frog bladder connected with transducer was inserted into the gallbladder to record the gallbladder pressure. Microinjection of OT (10-50 nmol/L, 100 nl) dose dependently increased the strength of gallbladder phasic contraction. The excitatory effect of OT (10 nmol/L, 100 nl) was completely abolished by atosiban (10 mmol/L, 100 nl), the specific OT receptor antagonist, but was not influenced by [deamino-Pen(1), O-Me-Tyr(2),Arg(8)]-vasopressin (10 mmol/L, 100 nl), the V(1) receptor antagonist. Pretreatment of ketamine (10 mmol/L, 100 nl), the NMDA receptor antagonist, suppressed the gallbladder motor response to OT; but pretreatment of 6-Cyaon-7-Nitroquinoxaline-2,3-(1H,4H)-Dione (CNQX; 10 mmol/L, 100 nl), the non-NMDA receptor antagonist, did not affect it. Pretreatment of L-NAME (10 mmol/L, 100 nl), the nitric oxide synthase (NOS) inhibitor, or methyl blue (10 mmol/L, 100 nl), the guanylyl cyclase inhibitor, inhibited the excitatory effect of OT on gallbladder motility. Hence, we deduced that the microinjection of OT into the DMV enhanced the gallbladder motility through binding specific OT receptors and activating the NMDA receptor-NO-cGMP pathway.
- Subjects :
- 6-Cyano-7-nitroquinoxaline-2,3-dione pharmacology
Analysis of Variance
Animals
Benzenesulfonates pharmacology
Cyclic GMP antagonists & inhibitors
Deamino Arginine Vasopressin pharmacology
Dose-Response Relationship, Drug
Drug Interactions
Female
Gallbladder drug effects
Gallbladder innervation
Gastrointestinal Motility physiology
Ketamine pharmacology
Male
NG-Nitroarginine Methyl Ester pharmacology
Nitric Oxide antagonists & inhibitors
Oxytocin antagonists & inhibitors
Rabbits
Receptors, N-Methyl-D-Aspartate antagonists & inhibitors
Renal Agents pharmacology
Time Factors
Vagus Nerve physiology
Vasotocin pharmacology
Cyclic GMP physiology
Gastrointestinal Motility drug effects
Medulla Oblongata drug effects
Nitric Oxide physiology
Oxytocin pharmacology
Receptors, N-Methyl-D-Aspartate physiology
Vasotocin analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 0006-8993
- Volume :
- 1032
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Brain research
- Publication Type :
- Academic Journal
- Accession number :
- 15680949
- Full Text :
- https://doi.org/10.1016/j.brainres.2004.10.057