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Persistent borna disease virus infection confers instability of HSP70 mRNA in glial cells during heat stress.
- Source :
-
Journal of virology [J Virol] 2005 Feb; Vol. 79 (4), pp. 2033-41. - Publication Year :
- 2005
-
Abstract
- Borna disease virus (BDV) is a highly neurotropic RNA virus that causes neurological disorders in many vertebrate species. Although BDV readily establishes lasting persistence, persistently infected cells maintain an apparently normal cell phenotype in terms of morphology, viability, and proliferation. In this study, to understand the regulation of stress responses in BDV infection, we investigated the expression of heat shock proteins (HSPs) in glial cells persistently infected with BDV. Interestingly, we found that BDV persistence did not upregulate HSP70 expression even in cells treated with heat stress. Furthermore, BDV-infected glial cells exhibited rapid rounding and detachment from the culture plate under various stressful conditions. Immunofluorescence analysis demonstrated that heat stress rapidly disrupts the cell cytoskeleton only in persistently infected cells, suggesting a lack of thermotolerance. Intriguingly, we found that although persistently infected glial cells expressed HSP70 mRNA after heat stress, its expression rapidly disappeared during the recovery period. These observations indicated that persistent BDV infection may affect the stability of HSP70 mRNA. Finally, we found that the double-stranded RNA-dependent protein kinase (PKR) is expressed at a constant level in persistently infected cells with or without heat shock. Considering the interrelationship between HSP70 and PKR production, our data suggest that BDV infection disturbs the cellular stress responses to abolish antiviral activities and maintain persistence.
- Subjects :
- Animals
Borna Disease pathology
Cell Line
Gene Expression Regulation, Viral
HSP70 Heat-Shock Proteins genetics
Hot Temperature
Neuroglia cytology
Protein Kinases metabolism
RNA, Double-Stranded metabolism
RNA, Messenger metabolism
Rats
Temperature
Transcription, Genetic
Borna Disease metabolism
Borna disease virus
HSP70 Heat-Shock Proteins metabolism
Neuroglia virology
Stress, Physiological metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0022-538X
- Volume :
- 79
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 15681405
- Full Text :
- https://doi.org/10.1128/JVI.79.4.2033-2041.2005