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[Determination of primary structure of a novel peptide from mistletoe and its antitumor activity].
- Source :
-
Yao xue xue bao = Acta pharmaceutica Sinica [Yao Xue Xue Bao] 2004 Oct; Vol. 39 (10), pp. 813-7. - Publication Year :
- 2004
-
Abstract
- Aim: To study the antitumor peptide components in the stems and leaves of mistletoe (Viscum coloratum (Kom.) Nakai), the primary structure of the novel peptide was elucidated.<br />Methods: Cation exchange, gel filtration and HPLC were employed for isolation and purification. Matrix Assisted Laser Desorption Ionization-Time of Flight-Mass Spectrometry was used to determine the mass. The complete amino acid sequence of the novel peptide was obtained by Edman degradation combined with enzyme digestion. The antitumor activity of the peptide in vitro was studied with MTT method.<br />Results: The primary stucture of the peptide named as viscotoxin B2 is KSCCKNTTGRNIYNTCRFAGGSRERCAKLSGCKIISASTCPSDYPK. The IC50 value of viscotoxin B2 on the Rat Osteoblast-like Sarcoma 17/2.8 cells in vitro is 1.6 mg x L(-1).<br />Conclusion: Viscotoxin B2 in V. coloratum, which has high similarity with viscotoxins from V. album, showed antitumor activity.
- Subjects :
- Amino Acid Sequence
Animals
Antineoplastic Agents, Phytogenic chemistry
Antineoplastic Agents, Phytogenic pharmacology
Bone Neoplasms pathology
Inhibitory Concentration 50
Molecular Sequence Data
Molecular Weight
Osteosarcoma pathology
Peptides isolation & purification
Peptides pharmacology
Plant Leaves chemistry
Plant Proteins isolation & purification
Plant Stems chemistry
Rats
Tumor Cells, Cultured drug effects
Antineoplastic Agents, Phytogenic isolation & purification
Peptides chemistry
Plant Proteins chemistry
Plant Proteins pharmacology
Plants, Medicinal chemistry
Viscum chemistry
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0513-4870
- Volume :
- 39
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Yao xue xue bao = Acta pharmaceutica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 15700822