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Identification of an antifungal chitinase from a potential biocontrol agent, Bacillus cereus 28-9.
- Source :
-
Journal of biochemistry and molecular biology [J Biochem Mol Biol] 2005 Jan 31; Vol. 38 (1), pp. 82-8. - Publication Year :
- 2005
-
Abstract
- Bacillus cereus 28-9 is a chitinolytic bacterium isolated from lily plant in Taiwan. This bacterium exhibited biocontrol potential on Botrytis leaf blight of lily as demonstrated by a detached leaf assay and dual culture assay. At least two chitinases (ChiCW and ChiCH) were excreted by B. cereus 28-9. The ChiCW-encoding gene was cloned and moderately expressed in Escherichia coli DH5alpha. Near homogenous ChiCW was obtained from the periplasmic fraction of E. coli cells harboring chiCW by a purification procedure. An in vitro assay showed that the purified ChiCW had inhibitory activity on conidial germination of Botrytis elliptica, a major fungal pathogen of lily leaf blight.
- Subjects :
- Amino Acid Sequence
Bacillus cereus genetics
Chitinases isolation & purification
Escherichia coli genetics
Escherichia coli metabolism
Lilium enzymology
Lilium genetics
Molecular Sequence Data
Plant Diseases microbiology
Sequence Homology, Amino Acid
Taiwan
Antifungal Agents pharmacology
Bacillus cereus enzymology
Chitinases genetics
Chitinases metabolism
Pest Control, Biological
Subjects
Details
- Language :
- English
- ISSN :
- 1225-8687
- Volume :
- 38
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of biochemistry and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 15715951
- Full Text :
- https://doi.org/10.5483/bmbrep.2005.38.1.082