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WRKY group IId transcription factors interact with calmodulin.
- Source :
-
FEBS letters [FEBS Lett] 2005 Feb 28; Vol. 579 (6), pp. 1545-50. - Publication Year :
- 2005
-
Abstract
- Calmodulin (CaM) is a ubiquitous Ca(2+)-binding protein known to regulate diverse cellular functions by modulating the activity of various target proteins. We isolated a cDNA encoding AtWRKY7, a novel CaM-binding transcription factor, from an Arabidopsis expression library with horseradish peroxidase-conjugated CaM. CaM binds specifically to the Ca(2+)-dependent CaM-binding domain (CaMBD) of AtWRKY7, as shown by site-directed mutagenesis, a gel mobility shift assay, a split-ubiquitin assay, and a competition assay using a Ca2+/CaM-dependent enzyme. Furthermore, we show that the CaMBD of AtWRKY7 is a conserved structural motif (C-motif) found in group IId of the WRKY protein family.
- Subjects :
- Amino Acid Sequence
Arabidopsis chemistry
Arabidopsis classification
Arabidopsis genetics
Arabidopsis metabolism
Arabidopsis Proteins chemistry
Arabidopsis Proteins genetics
Arabidopsis Proteins isolation & purification
Calmodulin-Binding Proteins chemistry
Calmodulin-Binding Proteins classification
Calmodulin-Binding Proteins genetics
Calmodulin-Binding Proteins metabolism
Molecular Sequence Data
Peptide Fragments chemistry
Peptide Fragments genetics
Peptide Fragments metabolism
Phylogeny
Protein Binding
Sequence Alignment
Transcription Factors chemistry
Transcription Factors genetics
Transcription Factors isolation & purification
Ubiquitin metabolism
Arabidopsis Proteins metabolism
Calmodulin metabolism
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 579
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 15733871
- Full Text :
- https://doi.org/10.1016/j.febslet.2005.01.057