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NELIN, a new F-actin associated protein, stimulates HeLa cell migration and adhesion.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2005 May 20; Vol. 330 (4), pp. 1127-31. - Publication Year :
- 2005
-
Abstract
- A new gene (GenBank Accession No. AF114264) was cloned from umbilical vein wall tissue by using RT-PCR. The gene shares high similarity to the gene encoding F-actin binding protein nexilin, so named as NELIN. A clone of 2737bp contains open reading frame of 1344bp extending from 412 to 1755. NELIN was expressed primarily in the heart and skeletal muscle among eight tested normal tissues. Immunofluorescence and immunoprecipitation demonstrated that NELIN product was associated with F-actin. Stable transfection of NELIN into HeLa cells increased the cell migration by 2.17-fold and the adhesion by 1.67-fold, respectively, compared to cells with the empty vector (P<0.05). The results support that NELIN product is an F-actin associated protein and mediates cell motility.
- Subjects :
- Actins metabolism
Base Sequence
Carrier Proteins genetics
Carrier Proteins metabolism
Cloning, Molecular
HeLa Cells
Humans
Microfilament Proteins genetics
Microfilament Proteins metabolism
Molecular Sequence Data
Organ Specificity
Carrier Proteins physiology
Cell Adhesion physiology
Cell Movement physiology
Microfilament Proteins physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 330
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 15823560
- Full Text :
- https://doi.org/10.1016/j.bbrc.2005.03.082