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[Preparation of a monoclonal antibodies against Plasmodium falciparum glutamate dehydrogenase and establishment of colloidal gold-immunochromatographic assay].
- Source :
-
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA [Di Yi Jun Yi Da Xue Xue Bao] 2005 Apr; Vol. 25 (4), pp. 435-8. - Publication Year :
- 2005
-
Abstract
- Objective: To prepare a monoclonal antibodies (mAbs) against glutamate dehydrogenase (GDH) of Plasmodium falciparum (FCC1/HN strain) and establish colloidal gold-immunochromatographic assay (GICA) for diagnosis of Plasmodium falciparum malaria.<br />Methods: Recombinant GDH was used to immunize Balb/C mice and the mAbs against GDH were prepared using hybridoma technique followed by identification of IgG isotype and its affinity. Protein-G affinity chromatography was employed to purify the antibodies, which were labeled with colloidal gold for establishment of GICA for Plasmodium falciparum detection.<br />Results: Six mAbs were obtained and identified as IgG1(kappa) of IgG isotypes with affinity constants (Kaff) ranging from 1 x 10(-8) to 2.8 x 10(-10). GICA had a sensitivity of 86.66%; and specificity of 96.43%; for Plasmodium falciparum detection compared with routine microscopic examination.<br />Conclusion: The established GICA is rapid and accurate for Plasmodium falciparum detection with such potential utility as for instant diagnosis of Plasmodium falciparum malaria.
- Subjects :
- Animals
Chromatography, High Pressure Liquid methods
Female
Gold Colloid
Immunoassay methods
Malaria, Falciparum parasitology
Mice
Mice, Inbred BALB C
Plasmodium falciparum enzymology
Plasmodium falciparum immunology
Antibodies, Monoclonal biosynthesis
Antibodies, Protozoan analysis
Glutamate Dehydrogenase immunology
Malaria, Falciparum diagnosis
Plasmodium falciparum isolation & purification
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1000-2588
- Volume :
- 25
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
- Publication Type :
- Academic Journal
- Accession number :
- 15837649