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Interaction of Histoplasma capsulatum with human macrophages, dendritic cells, and neutrophils.
Interaction of Histoplasma capsulatum with human macrophages, dendritic cells, and neutrophils.
- Source :
-
Methods in molecular medicine [Methods Mol Med] 2005; Vol. 118, pp. 181-91. - Publication Year :
- 2005
-
Abstract
- Histoplasma capsulatum (Hc) is a dimorphic fungal pathogen indigenous to the Ohio and Mississippi River Valleys in the United States. Infection is initiated by inhalation of microconidia or small mycelial fragments into the terminal bronchioles of the lung. The conidia are taken up by alveolar macrophages (Mphi), in which they convert to the pathogenic yeast phase. The yeasts replicate in the alveolar Mphi and other Mphi recruited to the lung as part of the inflammatory response. Thus, the yeasts are able to disseminate from the lung to other organs, such as the liver and spleen. As a facultative intracellular parasite, the interaction of Hc yeasts with Mphi is a critical component of the host response to infection. In addition, Hc yeasts have critical interactions with inflammatory neutrophils, and with dendritic cells (DCs) in the lung and other organs. Indeed, recent new evidence suggests that DCs may be the key antigen-presenting cells that initiate cell-mediated immunity. Thus, the methods described in this chapter cover quantitation of the binding, ingestion, and intracellular replication of Hc yeasts in human Mphi, DCs, and neutrophils.
- Subjects :
- Antigen Presentation
Dendritic Cells immunology
Histoplasma growth & development
Histoplasma immunology
Humans
Immunity, Cellular
Immunologic Techniques
In Vitro Techniques
Macrophages immunology
Mycology methods
Neutrophils immunology
Phagocytosis
Dendritic Cells microbiology
Histoplasma pathogenicity
Macrophages microbiology
Neutrophils microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1543-1894
- Volume :
- 118
- Database :
- MEDLINE
- Journal :
- Methods in molecular medicine
- Publication Type :
- Academic Journal
- Accession number :
- 15888943
- Full Text :
- https://doi.org/10.1385/1-59259-943-5:181