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Clonal breadth of the HIV-1-specific T-cell receptor repertoire in vivo as determined by subtractive analysis.
- Source :
-
AIDS (London, England) [AIDS] 2005 Jun 10; Vol. 19 (9), pp. 887-96. - Publication Year :
- 2005
-
Abstract
- Objective: Although the epitopic breadth of HIV-1-specific CD8 T lymphocyte (CTL) responses has been described, the T cell receptor (TCR) diversity of virus-specific cells remains poorly defined.<br />Design and Methods: To address this issue, we applied a novel technique for subtractive analysis of the HIV-1-specific CTL repertoire, combining specific deletion of peptide-specific cells by 5-fluorouracil with TCR spectratyping to identify clonal breadth of CTL recognizing individual peptides.<br />Results: Comprehensive analysis of an infected individual reveals that nine identified HIV-1-specific responses are comprised of at least 38 distinct T-cell clones (ranging from two to 10 distinct clones per epitope).<br />Conclusion: Given the potentially crucial role of T-cell receptor breadth for viral recognition and avoidance of escape, this subepitopic analysis of CTL may offer an important measure of cellular immunity for pathogenesis and vaccine studies.
- Subjects :
- Clonal Deletion
Clone Cells immunology
Complementarity Determining Regions immunology
Cytotoxicity, Immunologic
Enzyme-Linked Immunosorbent Assay methods
Epitopes, T-Lymphocyte immunology
Flow Cytometry methods
Humans
Immunity, Cellular
Male
Receptors, Antigen, T-Cell, alpha-beta immunology
Reverse Transcriptase Polymerase Chain Reaction methods
CD8-Positive T-Lymphocytes immunology
HIV Infections immunology
HIV-1
Receptors, Antigen, T-Cell immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0269-9370
- Volume :
- 19
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- AIDS (London, England)
- Publication Type :
- Academic Journal
- Accession number :
- 15905669
- Full Text :
- https://doi.org/10.1097/01.aids.0000171402.00372.c2