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Biosynthesis and cell-wall deposition of a pectin-xyloglucan complex in pea.

Authors :
Cumming CM
Rizkallah HD
McKendrick KA
Abdel-Massih RM
Baydoun EA
Brett CT
Source :
Planta [Planta] 2005 Oct; Vol. 222 (3), pp. 546-55. Date of Electronic Publication: 2005 May 24.
Publication Year :
2005

Abstract

Golgi-enriched enzyme preparations prepared from etiolated pea epicotyls incorporated [U-(14)C]galactose from UDP-[U-(14)C]galactose into the 1,4-beta-galactan sidechains of a pectin-xyloglucan complex. This complex could bind to paper and was degraded both by pectin-degrading enzymes and by a xyloglucan-specific endoglucanase. Gel permeation chromatography was used to assess the molecular size of the complex and of enzymically-degraded, galactan-containing fragments of it. Etiolated pea stems were labelled with [U-(14)C]sucrose for 1 h, and the newly-synthesised cell wall polysaccharides were extracted with EDTA or NaOH and fractionated by ion-exchange chromatography. The NaOH-extracted, acidic radioactive polysaccharides obtained in this way were also degraded both by pectin-degrading enzymes and by xyloglucan-specific endoglucanase. Analysis of the radioactive sugar composition indicated that neutral sugars characteristic of both pectin and xyloglucan were present. Analysis of the total non-radioactive, neutral sugar composition of the NaOH-extracted, acidic cell-wall polysaccharides indicated that pectin-xyloglucan complexes were a general feature of the cell wall in this tissue.

Details

Language :
English
ISSN :
0032-0935
Volume :
222
Issue :
3
Database :
MEDLINE
Journal :
Planta
Publication Type :
Academic Journal
Accession number :
15912355
Full Text :
https://doi.org/10.1007/s00425-005-1560-2