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Biosynthesis and cell-wall deposition of a pectin-xyloglucan complex in pea.
- Source :
-
Planta [Planta] 2005 Oct; Vol. 222 (3), pp. 546-55. Date of Electronic Publication: 2005 May 24. - Publication Year :
- 2005
-
Abstract
- Golgi-enriched enzyme preparations prepared from etiolated pea epicotyls incorporated [U-(14)C]galactose from UDP-[U-(14)C]galactose into the 1,4-beta-galactan sidechains of a pectin-xyloglucan complex. This complex could bind to paper and was degraded both by pectin-degrading enzymes and by a xyloglucan-specific endoglucanase. Gel permeation chromatography was used to assess the molecular size of the complex and of enzymically-degraded, galactan-containing fragments of it. Etiolated pea stems were labelled with [U-(14)C]sucrose for 1 h, and the newly-synthesised cell wall polysaccharides were extracted with EDTA or NaOH and fractionated by ion-exchange chromatography. The NaOH-extracted, acidic radioactive polysaccharides obtained in this way were also degraded both by pectin-degrading enzymes and by xyloglucan-specific endoglucanase. Analysis of the radioactive sugar composition indicated that neutral sugars characteristic of both pectin and xyloglucan were present. Analysis of the total non-radioactive, neutral sugar composition of the NaOH-extracted, acidic cell-wall polysaccharides indicated that pectin-xyloglucan complexes were a general feature of the cell wall in this tissue.
- Subjects :
- Cellulase metabolism
Galactosidases metabolism
Glucans chemistry
Pectins chemistry
Plant Stems cytology
Plant Stems metabolism
Polygalacturonase metabolism
Time Factors
Xylans chemistry
Cell Wall metabolism
Glucans metabolism
Pisum sativum cytology
Pisum sativum metabolism
Pectins metabolism
Xylans metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0032-0935
- Volume :
- 222
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Planta
- Publication Type :
- Academic Journal
- Accession number :
- 15912355
- Full Text :
- https://doi.org/10.1007/s00425-005-1560-2