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Migrating postmitotic neural precursor cells in the ventricular zone extend apical processes and form adherens junctions near the ventricle in the developing spinal cord.
- Source :
-
Neuroscience research [Neurosci Res] 2005 Jul; Vol. 52 (3), pp. 250-62. Date of Electronic Publication: 2005 Apr 26. - Publication Year :
- 2005
-
Abstract
- Postmitotic neural precursors are generated in the ventricular zone (VZ) of the developing neural tube and immediately migrate to the mantle layer (ML) where they differentiate into mature neurons. Although the regulation of neuronal differentiation and migration has extensively been studied, the behavior of the early postmitotic precursors migrating toward the ML is largely unknown. In this study, we have identified Neph3 as a specific marker for early postmitotic neural precursors in the VZ of the developing spinal cord. Analysis of Neph3 localization by immunofluorescence and immunoelectron microscopy revealed that early neural precursors in the VZ possessed not only pia-connected processes but also ones that reached the ventricle. This apical extension of processes was confirmed by analyzing another early postmitotic marker, Dll1 mRNA, which was actively transported toward the ventricle and accumulated at the termini of the processes. Furthermore, adherens junctions (AJs) were formed around the apical end of processes extending from Neph3- and Dll1 mRNA-positive postmitotic precursors. Taken together, these observations suggest that migrating early postmitotic neural precursors in the VZ of the developing spinal cord form a neuroepithelial cell-like bipolar morphology and communicate with their neighboring cells through AJs.
- Subjects :
- Adherens Junctions ultrastructure
Animals
Blotting, Northern
Cadherins metabolism
Cell Aggregation physiology
Cells, Cultured
Cerebral Ventricles embryology
Cerebral Ventricles ultrastructure
Cloning, Molecular methods
DNA-Binding Proteins metabolism
Drosophila Proteins metabolism
Embryo, Mammalian
Gene Expression Regulation, Developmental physiology
Genetic Vectors physiology
Homeodomain Proteins metabolism
Immunohistochemistry methods
In Situ Hybridization methods
Membrane Proteins genetics
Membrane Proteins metabolism
Mice
Microscopy, Immunoelectron
Neurofilament Proteins metabolism
Neurons ultrastructure
Phosphoproteins metabolism
RNA, Messenger biosynthesis
Reverse Transcriptase Polymerase Chain Reaction methods
Spinal Cord embryology
Tubulin metabolism
Zonula Occludens-1 Protein
Adherens Junctions physiology
Cell Movement physiology
Cerebral Ventricles cytology
Neurons physiology
Spinal Cord cytology
Stem Cells physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0168-0102
- Volume :
- 52
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Neuroscience research
- Publication Type :
- Academic Journal
- Accession number :
- 15927725
- Full Text :
- https://doi.org/10.1016/j.neures.2005.03.014