Back to Search Start Over

Cobalt chloride induces neurite outgrowth in rat pheochromocytoma PC-12 cells through regulation of endothelin-2/vasoactive intestinal contractor.

Cobalt chloride induces neurite outgrowth in rat pheochromocytoma PC-12 cells through regulation of endothelin-2/vasoactive intestinal contractor.

Authors :
Kotake-Nara E
Takizawa S
Quan J
Wang H
Saida K
Source :
Journal of neuroscience research [J Neurosci Res] 2005 Aug 15; Vol. 81 (4), pp. 563-71.
Publication Year :
2005

Abstract

We investigated whether endothelin-2/vasoactive intestinal contractor (ET-2/VIC) gene expression, upregulated by hypoxia in cancer cells, was associated with differentiation in neuronal cells. RT-PCR analysis, morphological observations, and immunostaining revealed that CoCl2, a hypoxic mimetic agent, at 200 microM increased expression of the ET-2/VIC gene, decreased expression of the ET-1 gene, and induced neurite outgrowth in PC-12 rat pheochromocytoma cells. These effects induced by 200 microM CoCl2 were completely inhibited by the antioxidant N-acetyl cysteine at 20 mM. In addition, CoCl2 increased the level of intracellular reactive oxygen species (ROS) at an early stage. Furthermore, interleukin (IL)-6 gene expression was upregulated upon the differentiation induced by CoCl2. These results suggest that expression of ET-2/VIC and ET-1 mediated by ROS may be associated with neuronal differentiation through the regulation of IL-6. When the cells were treated with 500 microM CoCl2 for 24 hr, however, ET-2/VIC gene expression disappeared, IL-6 gene expression was downregulated, and necrosis was subsequently induced in the PC-12 cells.<br /> (2005 Wiley-Liss, Inc.)

Details

Language :
English
ISSN :
0360-4012
Volume :
81
Issue :
4
Database :
MEDLINE
Journal :
Journal of neuroscience research
Publication Type :
Academic Journal
Accession number :
15948191
Full Text :
https://doi.org/10.1002/jnr.20568