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Differential astrocyte response to Theiler's murine encephalomyelitis virus infection.
- Source :
-
Intervirology [Intervirology] 2005; Vol. 48 (5), pp. 279-84. - Publication Year :
- 2005
-
Abstract
- Objectives: We aimed to address if selective astrocyte apoptosis is involved in the lack of murine demyelinating disease following infection by the L*-1 variant of Theiler's virus. In addition, we investigated whether L*-1-infected astrocytes were able to selectively express molecules whose effects would play a role as pathogenic factors.<br />Methods: Murine cultured astrocytes were infected with two Theiler viruses, the DA strain and the mutated DA variant L*-1, which does not synthesize the out of frame L* protein.<br />Results: Neither DA nor L*-1 provoked apoptosis, although they replicated in astrocytes inducing GFAP and iNOS expression, as well as subsequent nitric oxide production. In addition, both viruses caused an enhanced expression of ICAM-1, VCAM-1 and decay accelerating factor (DAF). In this connection, values of VCAM-1 and DAF induced by L*-1 were higher and lower, respectively, than those induced by DA.<br />Conclusions: Since no apoptosis was found, such mechanism would not be involved in the lack of TMEV-induced demyelinating disease by L*-1. In contrast, selective expression of VCAM-1 and DAF molecules induced by L*-1 could have a role in virus clearance from the central nervous system.<br /> (Copyright 2005 S. Karger AG, Basel)
- Subjects :
- Animals
Astrocytes metabolism
CD55 Antigens analysis
Cells, Cultured
Encephalomyelitis epidemiology
Gene Expression
Glial Fibrillary Acidic Protein analysis
Intercellular Adhesion Molecule-1 analysis
Mice
Nitric Oxide analysis
Nitric Oxide Synthase
Nitric Oxide Synthase Type II
Vascular Cell Adhesion Molecule-1 analysis
Apoptosis
Astrocytes pathology
Astrocytes virology
Theilovirus physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0300-5526
- Volume :
- 48
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Intervirology
- Publication Type :
- Academic Journal
- Accession number :
- 15956794
- Full Text :
- https://doi.org/10.1159/000085095