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Identification of plant glutaredoxin targets.
- Source :
-
Antioxidants & redox signaling [Antioxid Redox Signal] 2005 Jul-Aug; Vol. 7 (7-8), pp. 919-29. - Publication Year :
- 2005
-
Abstract
- Glutaredoxins (Grxs) are small ubiquitous proteins of the thioredoxin (Trx) family, which catalyze dithiol-disulfide exchange reactions or reduce protein-mixed glutathione disulfides. In plants, several Trx-interacting proteins have been isolated from different compartments, whereas very few Grx-interacting proteins are known. We describe here the determination of Grx target proteins using a mutated poplar Grx, various tissular and subcellular plant extracts, and liquid chromatography coupled to tandem mass spectrometry detection. We have identified 94 putative targets, involved in many processes, including oxidative stress response [peroxiredoxins (Prxs), ascorbate peroxidase, catalase], nitrogen, sulfur, and carbon metabolisms (methionine synthase, alanine aminotransferase, phosphoglycerate kinase), translation (elongation factors E and Tu), or protein folding (heat shock protein 70). Some of these proteins were previously found to interact with Trx or to be glutathiolated in other organisms, but others could be more specific partners of Grx. To substantiate further these data, Grx was shown to support catalysis of the stroma beta-type carbonic anhydrase and Prx IIF of Arabidopsis thaliana, but not of poplar 2-Cys Prx. Overall, these data suggest that the interaction could occur randomly either with exposed cysteinyl disulfide bonds formed within or between target proteins or with mixed disulfides between a protein thiol and glutathione.
- Subjects :
- Adenosine Triphosphate metabolism
Arabidopsis enzymology
Carbon Dioxide metabolism
Cell Respiration radiation effects
Citric Acid metabolism
Electron Transport
Glutaredoxins
Molecular Chaperones metabolism
Oxidative Stress
Oxygen metabolism
Peroxidases metabolism
Peroxiredoxins
Photochemistry
Polysaccharides biosynthesis
Polysaccharides metabolism
Protein Biosynthesis
Sulfur metabolism
Arabidopsis metabolism
Oxidoreductases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1523-0864
- Volume :
- 7
- Issue :
- 7-8
- Database :
- MEDLINE
- Journal :
- Antioxidants & redox signaling
- Publication Type :
- Academic Journal
- Accession number :
- 15998247
- Full Text :
- https://doi.org/10.1089/ars.2005.7.919