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Differentiation of Mycobacterium species by analysis of the heat-shock protein 65 gene (hsp65).
- Source :
-
International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2005 Jul; Vol. 55 (Pt 4), pp. 1649-1656. - Publication Year :
- 2005
-
Abstract
- The nucleotide sequences (604 bp) of partial heat-shock protein genes (hsp65) from 161 Mycobacterium strains containing 56 reference Mycobacterium species and 105 clinical isolates were determined and compared. hsp65 sequence analysis showed a higher degree of divergence between Mycobacterium species than did 16S rRNA gene analysis. Generally, the topology of the phylogenetic tree based on the hsp65 DNA sequences was similar to that of the 16S rRNA gene, thus revealing natural relationships among Mycobacterium species. When a direct sequencing protocol targeting 422 bp sequences was applied to 70 non-tuberculous mycobacterium (NTM) clinical isolates, all NTMs were clearly identified. In addition, an XhoI PCR restriction fragment length polymorphism analysis method for the differentiation of Mycobacterium tuberculosis complex from NTM strains was developed during this study. The results obtained suggest that 604 bp hsp65 sequences are useful for the phylogenetic analysis and species identification of mycobacteria.
- Subjects :
- Chaperonin 60
DNA, Bacterial analysis
DNA, Ribosomal analysis
Deoxyribonucleases, Type II Site-Specific metabolism
Humans
Molecular Sequence Data
Mycobacterium genetics
Mycobacterium tuberculosis classification
Mycobacterium tuberculosis genetics
Phylogeny
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Species Specificity
Bacterial Proteins genetics
Bacterial Typing Techniques
Chaperonins genetics
Mycobacterium classification
Subjects
Details
- Language :
- English
- ISSN :
- 1466-5026
- Volume :
- 55
- Issue :
- Pt 4
- Database :
- MEDLINE
- Journal :
- International journal of systematic and evolutionary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 16014496
- Full Text :
- https://doi.org/10.1099/ijs.0.63553-0