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Polarized helper T cells in tubercular pleural effusion: phenotypic identity and selective recruitment.
- Source :
-
European journal of immunology [Eur J Immunol] 2005 Aug; Vol. 35 (8), pp. 2367-75. - Publication Year :
- 2005
-
Abstract
- Containment of Mycobacterium tuberculosis critically depends on orchestrated generation of Th1 cells and their selective recruitment at the pathologic sites. Understanding the mechanism involved in this process is important for defining better intervention strategies. We investigated the surface phenotype of Th1 cells and the role of chemotactic factors in their selective recruitment in tuberculosis pleural effusion and tuberculin site. Memory T cells obtained from the pleural fluid expressed a battery of homing receptors such as CD11a, CCR5 and CXCR3. Similar expression profile was noted on T cells infiltrating the tuberculin site. Expression of their respective ligands such as ICAM-1, RANTES, MIP1-alpha, Mig and IP-10 were detected at pathologic sites. In vitro assay of T cell adherence to activated human umbilical vein endothelial cells (HUVEC) expressing chemotactic ligands suggests an important role of these homing molecules in their selective trafficking. Here, we demonstrate a hierarchy of CXCR3 in effector cell adhesion to HUVEC in vitro, although CD11a and CCR5 were also observed to mediate cell adhesion in an additive fashion. Findings of the present study provide mechanistic insights into the critical events of T cell trafficking in tuberculosis and may help designing better therapeutic modalities.
- Subjects :
- Adult
CD11 Antigens immunology
Cell Adhesion immunology
Cytokines metabolism
Endothelium, Vascular immunology
Female
Humans
Hypersensitivity, Delayed immunology
Ligands
Male
Pleural Effusion cytology
Pleural Effusion metabolism
Receptors, CCR5 immunology
Receptors, CXCR3
Receptors, Chemokine immunology
T-Lymphocyte Subsets immunology
T-Lymphocyte Subsets pathology
T-Lymphocytes, Helper-Inducer metabolism
Th1 Cells immunology
Tuberculosis, Pulmonary metabolism
Tuberculosis, Pulmonary pathology
Chemotaxis, Leukocyte immunology
Immunophenotyping
Pleural Effusion immunology
T-Lymphocytes, Helper-Inducer immunology
Tuberculosis, Pulmonary immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2980
- Volume :
- 35
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- European journal of immunology
- Publication Type :
- Academic Journal
- Accession number :
- 16025563
- Full Text :
- https://doi.org/10.1002/eji.200525977